子痫前期阴道念珠菌感染孕妇Th17/Treg及其相关细胞因子的表达  

Expression of Th17/Treg and related cytokines in pre-eclampsia pregnant with vaginal Candida infection

作　　者：郑敏红 李静 王淑虹 谢芳[4] ZHENG Min-hong;LI Jing;WANG Shu-hong;XIE Fang(Hongshan District Maternal and Child Health Hospital,Wuhan,Hubei 430070,China;不详)

机构地区：[1]武汉市洪山区妇幼保健院妇产科,湖北武汉430070 [2]武汉市第三医院妇产科,湖北武汉430074 [3]武汉市洪山区疾病预防控制中心检验科,湖北武汉430070 [4]武汉工程大学医院检验科,湖北武汉430074

出　　处：《中华医院感染学杂志》2022年第12期1829-1832,共4页Chinese Journal of Nosocomiology

基　　金：湖北省卫健委科研基金资助项目(WJ2019Z016)。

摘　　要：目的 探讨子痫前期(PE)阴道念珠菌感染孕妇Th17/Treg及其相关细胞因子水平的表达。方法 选取2020年3月-2021年3月武汉市洪山区妇幼保健院和武汉市第三医院收治的149例PE患者,按照阴道念珠菌感染情况分为感染组32例、非感染组117例。收集两组的临床资料;检测患者辅助性T17细胞(Th17)/调节性T细胞(Treg)及其特异性转录因子维甲酸相关孤核受体γt(RORγt)、叉头翼状螺旋转录因子(Foxp3)mRNA相对表达水平及血清白细胞介素(IL)-6、IL-10、IL-17、IL-21、IL-23、转化生长因子-β(TGF-β)各相关细胞因子水平。结果 感染组患者外周血Th17水平及Th17/Treg比值、RORγt mRNA相对表达水平、IL-6、IL-17、IL-21、IL-23及TGF-β水平高于非感染组,而Treg水平、Foxp3 mRNA相对表达水平、IL-10水平低于非感染组,差异有统计学意义(P<0.05)。结论 阴道念珠菌感染能影响患者Th17/Treg平衡及相关细胞因子水平的分泌,其与PE可能存在一定关联。OBJECTIVE To explore the expression levels of helper T17 cells(Th17)/regulatory T cells(Treg) and related cytokines in pre-eclampsia(PE) women with vaginal Candida infection. METHODS A total of 149 PE patients who were treated in Hongshan District Maternal and Child Health Hospital and Wuhan Third Hospital from Mar. 2020 to Mar. 2021 were enrolled in the study and divided into the infection group with 32 cases and the non-infection group with 117 cases according to the status of vaginal Candida infection. The clinical data were collected from the two groups. The relative expression levels of Th17/Treg and their specific transcription factors [retinoid-related orphan nuclear receptor γt(RORγt), forkhead/winged helix transcription factor p3(Foxp3)] mRNA as well as the levels of serum interleukin-6(IL-6), IL-10, IL-17, IL-21, IL-23 and transforming growth factor-β(TGF-β) were detected. RESULTS The peripheral blood Th17 level, Th17/Treg ratio, relative expression level of RORγt mRNA, and levels of IL-6, IL-17, IL-21, IL-23 and TGF-β of the infection group were higher than those of the non-infection group, while the Treg level, relative expression level of Foxp3 mRNA and IL-1 O level of the infection group were lower than those of the non-infection group, and there were significant differences(P<0.05). CONCLUSION The vaginal Candida infection may affect the balance of Th17/Treg and secretion of related cytokines, which may be associated with PE.

关 键 词：妊娠 阴道念珠菌感染 子痫前期 细胞因子 TH17/TREG 维甲酸相关孤核受体γt 叉头翼状螺旋转录因子 

分 类 号：R379.4[医药卫生—病原生物学]