异养硝化-好氧反硝化菌HNAD4的脱氮性能分析  

Denitrification Performance of Heterotrophic Nitrification-Aerobic Denitrification Bacteria HNAD4

作　　者：蒙小俊 祖德彪 张玉秀[2] 刘可欣 MENG Xiao-jun;ZU De-biao;ZHANG Yu-xiu;LIU Ke-xin(School of Tourism&Environment,Ankang University,Ankang 725000,China;School of Chemical&Environmental Engineering,China University of Mining and Technology-Beijing,Bejing 100083,China)

机构地区：[1]安康学院旅游与资源环境学院,陕西安康725000 [2]中国矿业大学<北京>化学与环境工程学院,北京100083

出　　处：《中国给水排水》2022年第15期29-36,共8页China Water & Wastewater

基　　金：国家水体污染控制与治理科技重大专项(2017ZX07402001);安康学院高层次人才科研专项(2016AYQDZR09)。

摘　　要：异养硝化-好氧反硝化为焦化废水处理革新提供了新思路。从某焦化废水处理厂活性污泥中分离获得一株高效的异养硝化-好氧反硝化菌株HNAD4,经16S rRNA鉴定为Pseudomonas sp.。分析了菌株HNAD4的硝化、反硝化以及同步硝化反硝化(SND)性能,研究了菌株对实际焦化废水的处理能力。结果表明,当菌株以柠檬酸钠为碳源、C/N为15、温度为35℃、pH为7.0时,硝化性能最优;以NO_(3)^(-)-N和NO_(2)^(-)-N为混合氮源时,两种氮源可同步去除,但反硝化优先利用NO_(3)^(-)-N;以NH_(4)^(+)-N、NO_(3)^(-)-N和NO_(2)^(-)-N为混合氮源时,SND过程中利用氮源的优先顺序依次为NH_(4)^(+)-N、NO_(3)^(-)-N和NO_(2)^(-)-N;当处理实际焦化废水时,与对照组相比,投加菌株的实验组可使A/O_(1)/O_(2)工艺中O_(1)池的NH_(4)^(+)-N去除率提高46.45%、TN去除率提高33.14%。Heterotrophic nitrification-aerobic denitrification provides a new idea for the innovation of coking wastewater treatment.An efficient heterotrophic nitrification-aerobic denitrification strain HNAD4 was isolated from the activated sludge of coking wastewater treatment plant and identified as Pseudomonas sp.by 16S rRNA.The nitrification,denitrification and simultaneous nitrification and denitrification(SND)characteristics of strain HNAD4 were analyzed,and the ability of the strain for actual coking wastewater treatment was investigated.The best nitrification performance was obtained when the following operating conditions were adopted:sodium citrate as carbon source,C/N ratio of 15,temperature of 35℃and pH of 7.0.When NO_(3)^(-)-N and NO_(2)^(-)-N were used as mixed nitrogen sources,the two nitrogen sources could be simultaneously removed,but NO_(3)^(-)-N was preferentially used in denitrification.When NH_(4)^(+)-N,NO_(2)^(-)-N and NO_(3)^(-)-N were used as mixed nitrogen sources,the priority of nitrogen sources utilized in SND process was NH_(4)^(+)-N,NO_(3)^(-)-N and NO_(2)^(-)-N.When the actual coking wastewater was treated,the removal efficiencies of NH_(4)^(+)-N and TN in O_(1)tank of A/O_(1)/O_(2)process increased by 46.45%and 33.14%in the experimental group compared with those in the control group.

关 键 词：焦化废水 异养硝化-好氧反硝化 同步硝化反硝化 生物脱氮 

分 类 号：TU992[建筑科学—市政工程]