某新冠肺炎定点医院环境气溶胶SARS-CoV-2核酸检测结果  

Result of SARS-CoV-2 nucleic acid test for aerosol in a designated COVID-19 hospital

作　　者：吴洪敏[1] 刘明星[2] 刘璇[3] 张芸[4] 王芳[5] 蔡昕芯 褚明[7] 秦艳秋[8] 蒋荣猛 茅一萍 WU Hong-min;LIU Ming-xing;LIU Xuan;ZHANG Yun;WANG Fang;CAI Xin-xin;CHU Ming;QIN Yan-qiu;JANG Rong-meng;MAO Yi-ping(Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212000,China;不详)

机构地区：[1]江苏大学附属医院感染管理科,江苏镇江212000 [2]苏州大学附属第一医院感染管理科,江苏苏州215000 [3]南京市第二医院检验科,江苏南京210029 [4]苏州大学第五人民医院感染管理科,江苏苏州215000 [5]南通市第三人民医院感染管理科,江苏南通226006 [6]南京鼓楼医院集团宿迁医院感染管理科,江苏宿迁223800 [7]江苏省人民医院心血管内科,江苏南京210029 [8]徐州市传染病医院感染管理科,江苏徐州221000 [9]首都医科大学附属北京地坛医院感染管理处,北京100015 [10]徐州医科大学附属医院感染管理科,江苏徐州221000

出　　处：《中华医院感染学杂志》2022年第9期1426-1429,共4页Chinese Journal of Nosocomiology

基　　金：首都卫生发展科研专项基金资助项目(2021-IG-2172)。

摘　　要：目的 对新冠肺炎定点医院隔离病区进行气溶胶采集和病毒核酸检测,为定点医院新冠医院感染防控工作提供科学依据。方法 于2021年8月14-24日,采用生物气溶胶采集器在某新冠肺炎定点医院隔离病区各布点区域进行空气气溶胶采集,所有标本同时采用荧光聚合酶链式扩增反应(PCR)和数字PCR两种方法进行新冠病毒核酸检测。结果 共采集空气气溶胶标本86份,荧光PCR检测结果全部为阴性,数字PCR检测结果显示,14份气溶胶标本检出新型冠状病毒核酸,检出率为16.28%。检出标本较集中的区域有病人卫生间和医务人员防护用品脱卸区;重症病房核酸检出率高于普通病房,但不同病区气溶胶病毒核酸检出率差异无统计学意义(χ^(2)=7.871,P=0.248),CT值>30和CT值≤30病人区域气溶胶病毒核酸检出率差异无统计学意义(χ^(2)=0.232,P=0.630)。结论 在新冠肺炎患者病程中晚期,隔离病区空气气溶胶仍有病毒核酸存在,但所有检出标本新冠病毒拷贝数均不高。室内拥挤、通风不良,且与病例有关联的环境中气溶胶病毒核酸检出率较高,医务人员应做好个人防护,保持环境通风良好,并做好环境清洁消毒和空气净化工作。OBJECTIVE To collect aerosol from isolation wards of a designated COVID-19 hospital and conduct the nucleic acid test so as to provide scientific basis for prevention and control of COVID-19. METHODS The air aerosol specimens were collected from layout sites in the isolation wards of the hospital by using bioaerosol collector, and the COVID-19 nucleic acid test was carried out for all of the specimens by using fluorescent polymerase chain reaction(PCR) and digital PCR. RESULTS A total of 86 aerosol samples were collected, all of which were tested negative for the fluorescent PCR, the result of the digital PCR test showed that 14 air aerosol samples were tested positive for COVID-19 nucleic acid, with the detection rate 16.28%. The toilets of the patients and taking-off area of protective supplies of healthcare workers were the major places where the specimens were tested positive. The positive rate of nucleic acid test was significantly higher in in intensive care units than in common wards, however, there was no significant difference in the positive rate of nucleic acid among the aerosol specimens in different wards(χ^(2)=7.871,P=0.248);there was no significant difference in the positive rate of nucleic acid of aerosol between the patients with CT value more than 30 and the patients with CT value no more than 30(χ^(2)=0.232,P=0.630). CONCLUSION There are still viral nucleic acids in the air aerosol of the isolation wards during the middle and late disease course of the COVID-19 patients, but the copy number of novel coronavirus is not large in the specimens. The detection rate of the viral nucleic acid is high in the aerosol of the wards that are crowed and poorly ventilated and are associated with the cases. It is necessary for the health care workers to take good care of themselves, keep the environment well ventilated and do a good job in environmental cleaning and disinfection and air purification.

关 键 词：气溶胶 新冠肺炎 隔离病房 荧光聚合酶链式扩增反应 数字聚合酶链式扩增反应 

分 类 号：R563.1[医药卫生—呼吸系统]