锻造态Mg-Zn-Zr/-Y镁合金体外生物相容性研究  

In Vitro Biocompatibility of Forged Mg-Zn-Zr/-Y Magnesium Alloy

作　　者：许东光 马春华 冷玉敏[1,3] 杨浩 XU Dongguang;MA Chunhua;LENG Yumin;YANG Hao(College of Mechanical and Electronic Engineering,Nanyang Normal University,Nanyang 473061,China;College of Materials Science and Engineering,Chongqing University,Chongqing 400044,China;Henan Rare-earth Alloy Material Engineering and Engineering Research Center,Nanyang 473061,China;Chemistry and Pharmaceutical Engineering,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院机电工程学院,河南南阳473061 [2]重庆大学材料科学与工程学院,重庆400044 [3]河南省稀土合金材料工程技术研究中心,河南南阳473061 [4]南阳师范学院化学与制药工程学院,河南南阳473061

出　　处：《生物化工》2022年第4期29-33,43,共6页Biological Chemical Engineering

基　　金：河南省科学技术厅科技攻关项目(212102210437)。

摘　　要：研究了锻造态Mg-Zn-Zr和Mg-Zn-Zr-Y镁合金在模拟体液中的pH值变化规律;按照ISO 10993的标准要求,分别制备了锻造态Mg-Zn-Zr/-Y合金不同浓度(100%、50%、10%)脐静脉血管内皮细胞(HUVEC)浸提液,通过体外溶血率测试了锻造态Mg-Zn-Zr/-Y镁合金的溶血率;通过CCK-8法体外细胞毒性试验及细胞形貌考察评价了锻造态Mg-Zn-Zr/-Y镁合金对HUVEC的细胞毒性。结果表明:锻造态Mg-Zn-Zr/-Y在SBF模拟体液中的腐蚀速度均高于纯镁的腐蚀速度;锻造态Mg-Zn-Zr镁合金溶血率为0.6%,锻造态Mg-Zn-Zr-Y镁合金溶血率为0.5%,不会导致严重溶血;锻造态Mg-Zn-Zr/-Y镁合金材料的浸提液浓度为10%时,对细胞的毒性较小,几乎不影响细胞的增殖。In this paper,the pH values of forged Mg-Zn-Zr/-Y magnesium alloys simulated body fluids(SBF)are studied.The forged Mg-Zn-Zr/-Y alloy is prepared with different concentrations(100%,50%,10%)human umbilical vein endothelial cell(HUVEC)extract according to the ISO 10993 standard,and the hemolysis rate of the forged magnesium alloy is measured by in vitro hemolysis rate.The forging state Mg-Zn-Zr/-Y isevaluated by CCK-8 in vitro cytotoxicity test and morphology observation cytotoxicity in HUVEC.The results show that the corrosion rate of forged Mg-Zn-Zr/-Y in SBF simulated body fluids is higher than that of pure magnesium.The hemolysis rate of Mg-Zn-Zr forged magnesium alloy is 0.6%,and which of forged Mg-Zn-Zr-Y magnesium alloy is 0.5%,indicating that forged magnesium alloy will not cause severe hemolysis.Forged Mg-Zn-Zr/-Y magnesium alloy materials with a concentration of 10%,the toxicity to cells is small.

关 键 词：锻造态Mg-Zn-Zr/-Y镁合金 溶血率 细胞毒性 细胞形貌 

分 类 号：R318.08[医药卫生—生物医学工程]