胰岛素样生长因子-1预处理对原代心肌细胞缺氧损伤的保护作用  

Protective Effect of Insulin-Like Growth Factor-1 Pretreatment on Hypoxic Injury of Primary Myocardial Cells

作　　者：吴连连 胡安康 WU Lianlian;HU Ankang(Laboratory Animal Center,Xuzhou Medical College,Xuzhou 221004,China)

机构地区：[1]徐州医科大学实验动物中心,江苏徐州221004

出　　处：《生物化工》2022年第4期66-69,共4页Biological Chemical Engineering

基　　金：江苏省高校自然科学基金(21KJB180011)。

摘　　要：目的:探究胰岛素样生长因子-1(IGF-1)对原代培养的心肌细胞缺氧损伤后的作用。方法:新生KM小鼠心肌细胞原代培养并通过α-Actin鉴定细胞形态。实验分组为正常组、缺氧组、IGF-1预处理组共3组,缺氧组细胞在更换无血清DMEM/F12培养基培养24 h后被置于缺氧培养箱中继续培养24 h,IGF-1预处理组在缺氧模型前1 h给予IGF-1。采用MTT法检测各组细胞的活力;应用比色法测定各组细胞上清液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果:缺氧后的心肌细胞活力降低(P<0.05),LDH、CK活性升高(P<0.05)、SOD活性下降(P<0.05),MDA含量升高(P<0.05);IGF-1预处理则显著提高了心肌细胞活力(P<0.05),并使LDH、CK活性降低(P<0.05),SOD活性升高(P<0.05),MDA含量降低(P<0.05)。结论:IGF-1预处理可以减轻缺氧对心肌细胞造成的损伤。Objective:The effect of insulin-like growth factor-1(IGF-1)on hypoxic injury of primary cultured cardiomyocytes is explored.Methods:Neonatal KM mouse cardiomyocytes are cultured in primary culture andα-Actin is used to identify cell morphology.The cells are divided into three groups:normal group,hypoxia group and IGF-1 pretreatment group.The cells in hypoxia group are cultured in serum-free DMEM/F12 medium for 24 hours and then placed in hypoxia incubator for 24 hours.IGF-1 pretreatment group is treated with IGF-11 hour before hypoxia model.MTT assay is used to detect the viability of cells in each group;The activity of lactate dehydrogenase(LDH),creatine kinase(CK),superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in cell supernatant are measured by colorimetry.Results:After hypoxia,the viability of cardiomyocytes is decreased(P<0.05),the activities of LDH and CK are increased(P<0.05),the activity of SOD is decreased(P<0.05),and the content of MDA is increased(P<0.05).IGF-1 pretreatment significantly increases the viability of cardiomyocytes(P<0.05),decreases the activities of LDH and CK(P<0.05),increases the activity of SOD(P<0.05),and decreases the content of MDA(P<0.05).Conclusion:IGF-1 pretreatment can reduce the injury of myocardial cells caused by hypoxia.

关 键 词：胰岛素样生长因子-1 心肌细胞 缺氧 乳酸脱氢酶 

分 类 号：Q28[生物学—细胞生物学]