丹皮酚通过JAK-STAT信号通路对宫颈癌细胞增殖迁移及侵袭的作用机制  

Mechanism of paeonol on the proliferation,migration and invasion of cervical cancer cells through JAK-STAT signaling pathway

作　　者：尹艳英[1] 石磊[1] 邹晓燕[1] YIN Yan-ying;SHI Lei;ZOU Xiao-yan(Daqing Oilfield General Hospital,Department of Gynaecology and Obstetrics,Daqing Heilongjiang 163000,China)

机构地区：[1]大庆油田总医院妇产科,黑龙江大庆163000

出　　处：《临床和实验医学杂志》2022年第16期1689-1693,共5页Journal of Clinical and Experimental Medicine

基　　金：黑龙江省医药卫生科学技术项目(编号:2018-073)。

摘　　要：目的分析丹皮酚通过Janus激酶(JAK)-信号传导和转录激活因子(STAT)信号通路对宫颈癌细胞(Hela细胞)增殖迁移及侵袭的作用机制。方法采用不同浓度丹皮酚对Hela细胞进行处理,根据丹皮酚浓度不同分为0、7.5、15.0、22.5、30.0、60.0 mg/L组,分别在培养24、48、72 h时采用MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Transwells小室检测细胞迁移和侵袭情况,并比较各组细胞培养72 h后的JAK1、JAK2、STAT3水平及其mRNA相对表达水平。结果使用不同浓度丹皮酚对Hela细胞处理24、48、72 h后,随处理时间的延长各组细胞吸光度(A)值、凋亡率均升高,细胞迁移个数、侵袭个数均增多,差异均有统计学意义(P<0.05);培养24、48、72 h时,7.5、15.0、22.5、30.0、60.0 mg/L组细胞A值、凋亡率均低于0 mg/L组,细胞迁移个数、侵袭个数均少于0 mg/L组,差异均有统计学意义(P<0.05),其中60 mg/L组细胞A值、凋亡率最低,细胞迁移个数、侵袭个数最少,7.5 mg/L组细胞A值、凋亡率最高,细胞迁移个数、侵袭个数最多,呈现剂量反应。使用不同浓度丹皮酚对Hela细胞处理24、48、72 h后,77.5、15.0、22.5、30.0、60.0 mg/L组JAK1、JAK2、STAT3水平及其mRNA相对表达水平均低于0 mg/L组,差异均有统计学意义(P<0.05),其中7.5 mg/L组JAK1、JAK2、STAT3水平及其mRNA相对表达水平最高,60.0 mg/L组JAK1、JAK2、STAT3水平及其mRNA相对表达水平最低,呈现剂量反应。结论丹皮酚可通过影响JAK-STAT信号通路而抑制宫颈癌细胞增殖、迁移和侵袭,促进细胞凋亡,且高剂量丹皮酚的作用效果更佳。Objective To analyze the mechanism of paeonol on the proliferation,migration and invasion of cervical cancer cells through Janus kinase(JAK)-signal transduction and transcription activator(STAT)signaling pathway.Methods Hela cells were treated with different concentrations of paeonol and were divided into 0 mg/L group,7.5 mg/L group,15.0 mg/L group,22.5 mg/L group,30.0 mg/L group and 60.0 mg/L group according to different concentrations of Paeonol.Cell proliferation was detected by MTT method at 24,48 and 72 h,respectively.Cell apoptosis was detected by flow cytometry,cell migration and invasion were detected by Transwells chamber,and the levels of JAK1,JAK2,STAT3 and their mRNA relative expression levels were compared in each group after 72 h culture.Results After treating Hela cells with different concentrations of paeonol for 24,48 and 72 h,absorbance(A)value and apoptosis rate of cells in each group increased with the extension of treatment time,and the number of cell migration and invasion increased,the differences were statistically significant(P<0.05).At 24,48 and 72 h,A value and apoptosis rate of cells in 7.5 mg/L group,15.0 mg/L group,22.5 mg/L group,30.0 mg/L group and 60.0 mg/L group were lower than those in 0 mg/L group,the number of cell migration and invasion was less than 0 mg/L group,the differences were statistically significant(P<0.05),the 60 mg/L group had the lowest cell A value and apoptosis rate,and the number of cell migration and invasion was the least,the 7.5 mg/L group had the highest cell A value and apoptosis rate,and the number of cell migration and invasion was the most,showing a dose response.After 24,48 and 72 h treatment with different concentrations of paeonol,the levels of JAK1,JAK2 and STAT3 and their mRNA relative expression in 7.5 mg/L,15.0 mg/L,22.5 mg/L,30.0 mg/L and 60.0 mg/L groups were all lower than those in 0 mg/L group,the differences were statistically significant(P<0.05),the levels of JAK1,JAK2 and STAT3 and their mRNA relative expression were the highest in 7.5 mg

关 键 词：宫颈癌细胞 丹皮酚 增殖 迁移 侵袭 JAK-STAT信号通路 

分 类 号：R285[医药卫生—中药学]