浙江省猪伪狂犬病病毒分离及其gD和gE基因遗传变异分析  

Isolation and phylogenetic analysis of gD and gE gene of pseudorabies virus in Zhejiang Province

作　　者：谢荣辉[1] 袁秀芳[2] 王雅婷 安慧婷 董建斐 黄靖 张璐 徐辉[1] 赵灵燕[1] XIE Ronghui;YUAN Xiufang;WANG Yating;AN Huiting;DONG Jianfei;HUANG Jing;ZHANG Lu;XU Hui;ZHAO Lingyan(Zhejiang Center of Animal Disease Control,Hangzhou 310018,China;Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省动物疫病预防控制中心,浙江杭州310018 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州310021

出　　处：《中国兽医学报》2022年第7期1327-1334,共8页Chinese Journal of Veterinary Science

基　　金：浙江省三农六方科技协作资助项目;浙江省重点研发计划资助项目(2021c02050);浙江省科技重点研发计划资助项目(2018C02028)。

摘　　要：对2018-2019年采自浙江省部分地区的30份猪伪狂犬病病毒阳性病料开展病毒分离鉴定,并进行分离毒株gD和gE基因克隆、测序和变异分析。结果显示,从30份样品中分离到11株猪伪狂犬病病毒;11个分离株之间的gD基因核苷酸及其推导氨基酸的同源性分别为99.3%~99.9%和98.0%~100.0%,gE基因核苷酸及其推导氨基酸的同源性分别为99.5%~99.9%和99.0%~99.8%;遗传进化分析显示,11株猪伪狂犬病病毒与我国2011年以后流行的毒株亲缘关系较近,与2011年前分离的国内毒株亲缘关系较远,与欧美毒株的亲缘关系更远;与疫苗株Bartha相比,11个分离株的gD和gE基因均存在碱基插入和点突变现象;gE基因推导氨基酸序列第48位均存在天冬氨酸的插入。本试验结果对于了解浙江省猪伪狂犬病病毒流行毒株的分子生物学特征和变异状况具有重要的意义。To study the genetic diversity and molecular characteristics of pseudorabies virus(PRV)in Zhejiang Province,isolation and variation analysis of gD and gE genes of PRV isolated from samples collected form Zhejiang Province were conducted.Eleven strains of PRV were isolated and their homologies of nucleotide and amino acid sequences of gD gene were 99.3%-99.9%and 98.0%-100.0%respectively.The homology of nucleotide and amino acid sequences of gE gene among 11 strains were 99.5%-99.9%and 99.0%-99.8%,respectively.Phylogenetic analysis of gD and gE gene revealed that the 11 strains of PRV were closely related to the isolates in China since 2011,while they were different from the strains in China before 2011,and the European and American strains.Compared with vaccine strain Bartha,base insertions and site mutations were revealed in gD and gE gene of the isolated strains.

关 键 词：猪伪狂犬病病毒 GD基因 GE基因 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]