Sequential One-Pot Three-Enzyme Synthesis of the Tetrasaccharide Repeating Unit of Group B Streptococcus Serotype Ⅷ Capsular Polysaccharide  

作　　者：Min Liang Wei Gong Chongzhen Sun Jielin Zhao Hong Wang Zonggang Chen Min Xiao Guofeng Gu 

机构地区：[1]National Glycoengineering Research Center,Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology,Shandong University,72 Binhai Road,Qingdao,Shandong 266237,China [2]School of Pharmaceutical Science,Shandong University,44 West Wenhua Road,Jinan,Shandong 250012,China [3]NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-based Medicine,Shandong University,Qingdao,Shandong 266237,china

出　　处：《Chinese Journal of Chemistry》2022年第9期1039-1044,共6页中国化学（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(Grant numbers 21877074 and 21672129).

摘　　要：The biochemical property and functional identification of three recombinant glycosyltransferases,includingβ-1,4-rhamnosyltransferase(Cps8R),β-1,4-galactosyltransferase(Cps8) and α-2,3-sialyltransferase(Cps8K)involved in the biosynthesis of the tetrasaccharide repeating unit of serotypeⅧcapsular polysaccharide(CPS)of Group B Streptococci(GBS),were systematically investigated.Subsequently,these recombinant enzymes were employed for one-pot three-enzyme efficient synthesis of the tetrasaccharide repeating unit of GBS serotypeⅧCPS using the chemically synthesized Glca-PP-(CH2)ir-OPh as the starting substrate in a satisfying yieldof 87%.

关 键 词：Streptococcus agalactiae Capsular polysaccharide OLIGOSACCHARIDES Enzyme catalysis BIOSYNTHESIS 

分 类 号：TQ426[化学工程]