核酸介导蛋白质共价修饰的研究进展  

Research Progress on Nucleic Acid-mediated Protein Covalent Modification

作　　者：杨雯 李婧影 杨黄浩 YANG Wen;LI Jing-ying;YANG Huang-hao(MOE Key Laboratory for Analytical Science of Food Safety and Biology,State Key Laboratory of Photocatalysis on Energy and Environment,College of Chemistry,Fuzhou University,Fuzhou 350108,China)

机构地区：[1]教育部食品安全与生物学分析科学重点实验室,能源与环境光催化国家重点实验室福州大学化学学院,福建福州350108

出　　处：《分析测试学报》2022年第9期1395-1402,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21974022)。

摘　　要：蛋白质是参与各种生理过程的关键生物分子。选择性的蛋白质化学修饰为开发新型生物制药和复杂生物系统中单个蛋白质的功能研究提供了有力工具。核酸作为一种多功能的分子工具,近十年被广泛用于构建选择性的蛋白质修饰策略。在这类策略中,核酸可以:(i)作为模板来辅助反应基团与蛋白质靠近,提高有效反应浓度;(ii)作为导向系统通过结合兴趣蛋白(Proteins of interest,POI)实现共价修饰的选择性;(iii)作为催化剂增强邻近区域的蛋白质修饰反应。该综述着重介绍核酸介导蛋白质共价标记策略的研究进展,并以不同的导向系统为分类,介绍了这类标记策略的发展及主要应用。Proteins are key biomolecules involved in a variety of physiological processes.Selective chemical modification of proteins provides powerful tools for the development of new classes of biopharmaceuticals and functional studies of individual protein in complex biological systems.Nucleic acid,a versatile molecular tool,has been widely used to construct selective protein modification strategies in the past decade.In such strategies,the nucleic acid can(i)serve as a template to assist the proximity of reactive groups to proteins,increasing the effective reaction concentration,and(ii)serve as a guidance system to achieve the selectivity of covalent conjugation by binding to proteins of interest(POI),and(iii)serve as a catalyst to enhance protein modification reactions in proximity.In this review,the progress of nucleic acid-mediated protein covalent labeling strategies is focused on,and the development and application of these strategies are described according to the classification of guidance systems.

关 键 词：核酸 蛋白质 选择性共价修饰 

分 类 号：O629.73[理学—有机化学] G353.11[理学—化学]