广东肇庆地区鸭疫里氏杆菌耐药性和毒力基因分析及ERIC-PCR分型研究  

Analysis of Drug Resistance, Virulence Gene Detection and ERIC-PCR Genotyping of Riemerella Anatipestifer in Zhaoqing City, Guangdong Province

作　　者：张旭财 李燕红 陆毅兴 常依 谢龙飞 曾振灵 熊文广 ZHANG Xucai;LI Yanhong;LU Yixing;CHANG Yi;XIE Longfei;ZENG Zhenling;XIONG Wenguang(Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,Guangzhou 510642,China;National Laboratory of Safety Evaluation(Environmental Assessment)of Veterinary Drugs,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria,Guangzhou 510642,China;Guangdong Provincial Laboratory of Modern Agricultural Science and Technology in Lingnan,Guangzhou 510642,China)

机构地区：[1]广东省兽药研制与安全评价重点实验室,广州510642 [2]国家兽药安全评价(环境评估)实验室(华南农大),广州510642 [3]国家兽医微生物耐药性风险评估实验室,广州510642 [4]岭南现代农业科学与技术广东省实验室,广州510642

出　　处：《中国动物传染病学报》2022年第4期39-47,共9页Chinese Journal of Animal Infectious Diseases

基　　金：广州市科技计划项目(202102020039)。

摘　　要：为了解广东省肇庆市鸭疫里氏杆菌的流行和耐药现状,本试验对当地4个养鸭场疑似传染性浆膜炎的病鸭进行细菌分离纯化、PCR鉴定,并对分离菌株进行了药敏试验、耐药基因、毒力基因检测及ERIC-PCR分型试验。药敏结果表明,14株鸭疫里氏杆菌对新霉素、安普霉素、恩诺沙星、替加环素、复方新诺明高度耐药,耐药率均为100%;对阿莫西林、头孢他啶和环丙沙星敏感度较高,敏感率分别为100%、100%和92.86%。耐药基因检测结果显示,所有分离菌均携带tet(X)和floR耐药基因,tet(B)和su l1耐药基因的检出率分别为64.29%和50%,其余9种耐药基因(aac(6′)-Ⅰb、aph(3')-Ⅱ、bla_(SHV)、bla_(DHA)、qnrA、qnr B、tet(A)、tet(C)、sul2)未被检出。毒力基因检测结果显示,AS87_04050、luxE和wza毒力基因的检出率较高,分别为100%、92.86%和100%,而TbdR 1均未被检出。ERIC-PCR分型试验结果表明,14株鸭疫里氏杆菌均成功分型,相似性在90%时,可分为7个基因型。本实验为广东省肇庆市鸭疫里氏杆菌病的治疗措施提供理论依据。In order to understand the prevalence and drug resistance status of Riemerella anatipestifer(RA)in Zhaoqing City,Guangdong Province,this experiment was conducted to isolate and purifi cation,PCR identifi cation from ducks suspected of infected with RA in four duck farms in Zhaoqing City,Guangdong Province.The isolates were also subjected to drug resistance test,resistance genes detection,virulence genes detection and ERIC-PCR typing test.The drug resistance results showed that 14 strains of RA were highly resistant to neomycin, apramycin, enrofl oxacin, tigecycline and trimethoprim, and the resistance rate was 100%, and showed more sensitive to amoxicillin, ceftazidime and ciprofl oxacin, and the sensitivity rate was as high with 100%, 100% and 92.86%, respectively. The results of drug resistance genes detection showed that all isolates carried tet (X) and fl o R resistance genes, and the detection rates of tet (B) and sul 1 resistance genes were 64.29% and 50%, respectively, while the remaining nine resistance genes(aac(6′) -Ⅰb, aph(3') -Ⅱ, bla_(SHV), bla_(DHA), qnr A, qnr B, tet (A), tet (C), sul 2)were not detected. The results of virulence genes detection showed that AS87 _04050, luxE and wza virulence genes were detected at high rates of 100%, 92.86% and 100%, respectively, while TbdR 1 was not detected at all. The results of ERIC-PCR typing test showed that all 14 strains of RA were successfully typed into 7 genotypes at 90% similarity. This experiment provides a theoretical basis for the therapeutic measures of RA in Zhaoqing City, Guangdong Province.

关 键 词：鸭疫里氏杆菌 耐药性 耐药基因 毒力基因 ERIC-PCR 

分 类 号：S852.612[农业科学—基础兽医学]