左旋咪唑对柔嫩艾美耳球虫活疫苗免疫增强效果的评价  

Levamisole Enhances Effi ciency of Live Vaccine of Eimeria tenella

作　　者：余水兰 赵其平[1] 朱顺海[1] 黄兵[1] 韩红玉[1] 于钰 梁珊珊 王青杰 王海霞 董辉[1] YU Shuilan;ZHAO Qiping;ZHU Shunhai;HUANG Bing;HAN Hongyu;YU Yu;LIANG Shanshan;WANG Qingjie;WANG Haixia;DONG Hui(Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]中国农业科学院上海兽医研究所农业农村部动物寄生虫学重点实验室,上海200241 [2]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国动物传染病学报》2022年第4期61-68,共8页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划(2017YFD0501205);国家寄生虫资源库(TDRC-2019-194-30)。

摘　　要：为评价左旋咪唑对柔嫩艾美耳球虫活疫苗的免疫增强效力,本研究以3日龄雏鸡为研究对象,先通过笼养试验筛选左旋咪唑的有效剂量,再通过平养试验验证其效果。笼养试验结果显示,0.1 mg和0.05 mg组免疫期间的增重均稍高于0.2 mg组及无佐剂免疫组,ACI分别高于或相当于无佐剂免疫组。平养试验结果显示,0.1 mg和0.05 mg组免疫期间的增重均稍好于无佐剂免疫组,0.10 mg组的ACI明显高于0.05 mg组,而后者与无佐剂免疫组的相当;0.1 mg组CD8水平于免疫后第14 d明显高于无佐剂免疫组,而0.05 mg组与无佐剂免疫组相当;0.1 mg和0.05 mg组的TNF-α水平在免疫后第21 d和攻虫后7 d均明显高于无佐剂免疫组,而CD4分子与无佐剂免疫组之间无显著性差异。以上结果表明,0.1 mg左旋咪唑可以明显提高柔嫩艾美耳球虫对3日龄雏鸡的免疫效果。本研究结果为进一步评估左旋咪唑作为球虫病活疫苗的免疫增强剂提供理论基础。In order to evaluate whether or not levamisole as an adjuvant improved effi cacy of live vaccine of Eimeria tenella,the effective doses were fi rstly screened with battery test and then verifi ed with fl oor-pen trial in 3-day-old chickens.The results of the battery test showed that the weight gains and ACI during administration of the vaccines containing 0.1 mg and 0.05 mg groups were slightly higher than those of 0.2 mg group and non-adjuvant group.The results of the fl oor-pen trial showed that the weight gains during use of the vaccines with 0.1 mg and 0.05 mg groups were slightly higher than those of non-adjuvant group,and the ACI of 0.10 mg group was signifi cantly higher than that of 0.05 mg group,of which was similar to the non-adjuvant group.In addition,the CD8 level of 0.1 mg group was signifi cantly higher than that of the non-adjuvant group on the 14th day post immunization while that of 0.05 group was similar to the non-adjuvant group. The TNF-α levels of 0.1 mg and 0.05 mg groups were signifi cantly higher than that of non-adjuvant group on the 21st day post immunization and 7th day post challenge. However, the CD4 levels of these two groups were similar to the non-adjuvant group. In conclusion, 0.1 mg levamisole signifi cantly improved the immune effect of live E. tenella vaccine in 3-day-old chickens, which provided preliminary data for further evaluation of levamisole as an immunopotentiator for live vaccines against coccidiosis.

关 键 词：左旋咪唑 柔嫩艾美耳球虫 免疫增强 

分 类 号：S858.31[农业科学—临床兽医学]