攀西地区牛肠道病毒遗传变异分析  

Analysis on the Genetic Variation of Bovine Enterovirus in Panxi Area

作　　者：杨佳欣 张涛 淦雨洁 许佳 王思芦[1] 郝桂英[1] 徐睿[1] 邓宇[1] YANG Jiaxin;ZHANG Tao;GAN Yujie;XU Jia;WANG Silu;HAO Guiying;Xu Rui;DENG Yu(School of Animal Science,Xichang College,Xichang 615000,China;Liangshan Animal Science Institute,Xichang 615000,China)

机构地区：[1]西昌学院动物科学学院,西昌615000 [2]凉山州畜科所,西昌615000

出　　处：《中国动物传染病学报》2022年第4期130-134,共5页Chinese Journal of Animal Infectious Diseases

基　　金：四川省科技厅应用基础研究面上项目(2018JY0243);四川省应用基础研究面上项目(2017JY0108);四川省教育厅自然科学重点项目(18ZAO442)。

摘　　要：为了解攀西地区牛肠道病毒(BEV)遗传进化关系,本研究采集新鲜牛粪便,应用RT-PCR方法对样品进行检测,对BEV分离株5'UTR进行克隆、测序,应用生物信息软件进行遗传进化关系分析。结果显示:攀西地区牛场BEV感染率达13.98%,其中E种BEV感染率为4.30%(4/93),F种BEV感染率为9.68%(9/93);同一奶牛场内存在E种和F种BEV感染;攀西地区BEV分离株与国内其他地区BEV毒株具有较高的同源性(73.1%~96.5%);攀西地区BEV分离株间同源性达75.5%~99.8%。本研究为攀西地区BEV流行情况与防控提供理论参考依据。To investigate the genetic evolution of bovine enterovirus(BEV)in Panxi region,fresh fecal samples were collected from 93 cattle and tested by RT-PCR using the specifi c primers based on the 5'untranslated region(5'UTR).The PCR products were cloned,sequenced and analyzed by the bioinformatics software.The results revealed a BEV positive rate of 13.98%(13/93),of which 4.30%(4/93)was enterovirus E and 9.68%(9/93)was enterovirus F.EV-E and EV-F infections were also detected in dairy cattle on the same farm.Genetic analysis revealed a homology of 75.5%to 99.8%among Panxi isolates and 73.1%-96.5%between Panxi isolates from Panxi and other areas in China.These results provided a useful reference for the epidemiological situation and prevention of BEV in Panxi region.

关 键 词：E种肠道病毒 F种肠道病毒 RT-PCR 5'UTR 

分 类 号：S852.65[农业科学—基础兽医学]