石菖蒲根茎化学成分及抗炎活性研究  

Chemical constituents from rhizoma of Acorus tatarinowii and its anti-inflammatory activity

作　　者：邱路雅 杨刚 金琼 罗晓东[4] 刘亚平 王易芬[1,2] QIU Lu-ya;YANG Gang;JIN Qiong;LUO Xiao-dong;LIU Ya-ping;WANG Yi-fen(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650201,China;Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]中国科学院昆明动物物研究所,云南昆明650201 [3]昆明理工大学食品科学与工程学院,云南昆明650500 [4]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201

出　　处：《中草药》2022年第15期4617-4624,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31872676);云南省应用基础研究面上项目(202101AT070436)。

摘　　要：目的 对石菖蒲Acorus tatarinowii干燥根茎的化学成分及其抗炎活性进行研究。方法 采用硅胶、YMC、Sephadex LH-20等多种柱色谱填料进行分离纯化,根据理化性质结合波谱数据鉴定化合物结构。通过检测脂多糖(lipopolysaccharide,LPS)诱导的RAW 264.7巨噬细胞中白细胞介素6(interleukin 6,IL-6)、IL-1β、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)炎症因子的表达和一氧化氮(NO)的抑制能力,来评估所分离化合物的抗炎作用。结果 从石菖蒲中分离得到18个单体化合物,包括9个苯丙素类、4个酚类、2个木脂素类、1个倍半萜、1个甾体及1个其他类型化合物,分别鉴定为原儿茶醛(1)、3,5-二甲氧基-4-羟基苯丙酮(2)、4-(乙氧基甲基)苯酚(3)、(2E)-3-(4-羟基-3-甲氧基苯基)-2-丙烯酸戊酯(4)、反式-阿魏酸二十二烷基酯(5)、反式-阿魏酸二十烷基酯(6)、4-羟基-3-甲氧基肉桂酸乙酯(7)、3-(2-甲氧基乙基)苯酚(8)、反式松柏醛(9)、对甲氧基苯酚(10)、β-细辛醚(11)、α-细辛醚(12)、2-乙酰基-菖蒲烯酮(13)、对羟基苯甲酸(14)、5,8,11,14,17-二十碳五烯酸(15)、(-)-丁香树脂(16)、(-)-松脂醇(17)、胡萝卜苷(18)。结论 化合物2~9均为首次从该属植物中分离获得。此外,单体化合物的抗炎活性与阳性对照组地塞米松比较,化合物1~4、6~12、14~16在20μg/mL质量浓度下能显著降低LPS诱导的RAW 264.7巨噬细胞中IL-6、IL-1β、TNF-α炎性细胞因子分泌和NO的释放,具有较好的体外抗炎作用。Objective To study the chemical compositions and their anti-inflammatory activities from the dried rhizoma of Acorus tatarinowii.Methods The ethyl acetate layer of A.tatarinowii was phytochemically investigated by multiple step chromatography techniques,such as silica gel,YMC,Sephadex LH-20 gel and other column chromatography techniques.The structures of these isolates were unambiguously elucidated by means of extensive spectroscopic and comparison with corresponding data of the reported literature.All the isolated compounds were evaluated for their anti-inflammatory effects via detecting inflammatory mediator releases(IL-6、IL-1β、TNF-α)and NO medium level in RAW 264.7 macrophage cells induced by lipopolysaccharide(LPS).Results A total of 18 compounds,isolated from ethyl acetate layer of A.tatarinowii,were identified as protocatechualdehyde(1),3,5-dimethoxy-4-hydroxypropiophenone(2),4-(ethoxymethyl)phenol(3),pentyl(2E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate(4),E-ferulic acid docosyl ester(5),E-ferulic acid eicosyl ester(6),4-hydroxy-3-methoxy cinnamic acid ethyl ester(7),3-(2-methoxyethyl)phenol(8),trans-coniferyl aldehyde(9),4-methoxyphenol(10),β-asarone(11),α-asarone(12),2-acetoxyacorenone(13),p-hydroxybenzoic acid(14),5,8,11,14,17-eicosapentaenoic acid(15),(-)-syringaresinol(16),(-)-pinoresinol(17)and daucosterol(18).Conclusion Among all the isolates,compounds 2—9 are isolated from Acorus species for the first time.Furthermore,compounds 1—4,6—12,14—16 could remarkably inhibited the secretion of inflammatory mediators(IL-6、IL-1β、TNF-α)and NO medium level in LPS-stimulated RAW 264.7 macrophages cells at the concentration of 20μg/m L,comparable to DXM used as the positive control.All the results suggested that A.tatarinowii possessed the anti-inflammatory activity.+.

关 键 词：石菖蒲 抗炎活性 炎症因子 3 5-二甲氧基-4-羟基苯丙酮 4-(乙氧基甲基)苯酚 反式松柏醛 

分 类 号：R284.1[医药卫生—中药学]