泽漆全草中二萜类成分研究  

Diterpenoids from whole herb of Euphorbia helioscopia

作　　者：王妍 梁旭博 赵珍珠 WANG Yan;LIANG Xu-bo;ZHAO Zhen-zhu(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046

出　　处：《中草药》2022年第15期4625-4633,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82003607);河南省高等学校重点科研项目(21A360002);河南省中医药科学研究专项(20-21ZY1039)。

摘　　要：目的 研究泽漆Euphorbia helioscopia全草的二萜类化学成分及其抗炎活性。方法 用醋酸乙酯对泽漆的95%乙醇提取物进行萃取得粗提物,然后采用大孔树脂、硅胶、中压制备色谱、SephadexLH-20和高效液相色谱等多种色谱技术对醋酸乙酯部位分离纯化,根据波谱数据及理化性质鉴定化合物结构。结果 从泽漆的醋酸乙酯部位分离得到20个二萜,分别鉴定为euphoscopin A(1)、euphoscopin B(2)、euphoscopin C(3)、euphoscopin E(4)、euphorbiapene D(5)、euphornin A(6)、euphornin B(7)、euphornin(8)、helioscopianoid M(9)、euphoheliosnoid D(10)、2α-hydroxy helioscopinolide B(11)、helioscopinolide A(12)、helioscopinolide B(13)、helioscopinolide C(14)、helioscopinolide D(15)、helioscopinolide H(16)、helioscopinolide L(17)、ent-16β,17-dihydroxyatlsan-3-one(18)、20-O-acetylingenol(19)、altotibetol(20)。并且所有化合物均筛选了NO生成抑制活性。结论 化合物15~17、20为首次从泽漆中分离得到,所有化合物均报道于大戟属的不同植物,说明大戟属植物合成二萜的酶系具有高度同源性;活性结果显示6、8~10和19显示了微弱的抗炎活性。Objective To investigate the diterpenoids from the whole herb of Euphorbia helioscopia. Methods The ethyl acetate extract of E. helioscopia extracted by EtOH(95%) was separated and purified by various chromatographic columns, including macroporous resins, silica gels, medium pressure liquid chromatography(MPLC), Sephadex LH-20, and preparative high performance liquid chromatography(prep-HPLC). The structures of purified compounds were determined by physiochemical properties and spectroscopic data. Results Twenty diterpenoids were isolated from of E. helioscopia, and they are identified as euphoscopin A(1), euphoscopin B(2), euphoscopin C(3), euphoscopin E(4), euphorbiapene D(5), euphornin A(6), euphornin B(7), euphornin(8), helioscopianoid M(9), euphoheliosnoid D(10), 2α-hydroxy helioscopinolide B(11), helioscopinolide A(12),helioscopinolide B(13), helioscopinolide C(14), helioscopinolide D(15), helioscopinolide H(16), helioscopinolide L(17),ent-16β,17-dihydroxyatlsan-3-one(18), 20-O-acetylingenol(19) and altotibetol(20). Additionally, all compounds were tested for their inhibitory activity against NO production. Conclusion All compounds were isolated from different plants of Euphorbia before, among which compounds 15, 16, 17, and 20 are isolated from E. helioscopia for the first time. And compounds 6, 8—10 and 19 showed weak anti-inflammatory activity.

关 键 词：泽漆 二萜 抗炎活性 euphornin A helioscopinolide D altotibetol 

分 类 号：R284.1[医药卫生—中药学]