民族药材飞蓬的质量标志物研究及资源品质评价  

Study on the quality markers and resource quality evaluation of folk medicines Erigeron acer

作　　者：李丽 张剑光 张英秀 苏学燕 张志锋 LI Li;ZHANG Jian-guang;ZHANG Ying-xiu;SU Xue-yan;ZHANG Zhi-feng(Institute of Qinghai-Tibetan Plateau,School of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Qinzhou Provincial Health School,Qinzhou 535000,China;Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China,Southwest Minzu University,Chengdu 610225,China;Sichuan Qiang Yi Medical Resources Protection and Utilization Technology Engineering Laboratory,Southwest Minzu University,Chengdu 610225,China)

机构地区：[1]西南民族大学青藏高原研究院药学院,四川成都610041 [2]钦州市卫生学校,广西钦州535000 [3]青藏高原民族药用资源保护与利用国家民委重点实验室,四川成都610225 [4]四川省羌彝药用资源保护与利用技术工程实验室,四川成都610225

出　　处：《中草药》2022年第15期4822-4832,共11页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(31870314);四川省科技厅区域创新合作项目(2020YFQ0007);西南民族大学中央高校基本科研业务专项基金项目(2021HQZZ01)。

摘　　要：目的 基于质量标志物(quality marker,Q-Marker)核心理论,从网络药理学(有效性)及化学成分(特有性、可测性)角度对民族药材飞蓬治疗心脑血管疾病的Q-Marker进行分析,评价其资源品质、寻找灯盏花素的新资源。方法 采用UPLC-Q-TOF-MS分析飞蓬的主要化学成分;通过网络药理学的有效性及化学成分特有性与可测性对飞蓬治疗心脑血管疾病Q-Marker进行筛选;采用UPLC-DAD法同时测定不同产地飞蓬Q-Marker含量。结果 从飞蓬中鉴定得到灯盏乙素、灯盏花苷I、异绿原酸、异绿原酸A、异绿原酸B、芹菜素、木犀草素、槲皮素等26个活性成分;初步确定绿原酸、灯盏花苷I、灯盏乙素、异绿原酸A、异绿原酸B为飞蓬治疗心血管疾病的Q-Marker;建立了基于UPLC-DAD测定飞蓬Q-Marker的方法,10批次不同产地的样品被测定,绿原酸质量分数为0.43~2.13mg/g,灯盏花苷I质量分数0.18~1.92mg/g,灯盏乙素质量分数为0.21~3.75 mg/g,绿原酸A质量分数为0.07~0.18 mg/g,异绿原酸B质量分数为0.56~1.76 mg/g。结论 该方法科学、可行,为飞蓬的质量控制研究提供了科学依据,同时为灯盏花素的新资源开发奠定基础。Objective Based on the core theories of quality marker(Q-Marker), network pharmacological(effectiveness) and chemical components(specificity and measurability) were developed to predict its Q-Marker, evaluate its quality and search for new resources of breviscapine. Methods The main chemical components in Erigeron acer were analyzed by UPLC-Q-TOF-MS;The Q-Marker of E. acer in the treatment of cerebrovascular diseases were discovered by network pharmacological effectiveness and chemical components specificity and measurability;Q-Marker of E. acer from different producing areas were simultaneously determined by UPLC-DAD. Results A total of 26 active compounds were identified, including scutellarin, erigeside I chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, apigenin, luteolin, quercetin and so on. The Q-Marker were chlorogenic acid,erigeside I, scutellarin, isochlorogenic acid A and isochlorogenic acid B. Ten batches of samples were investigated. Their contents were 0.43-2.13, 0.18-1.92, 0.21-3.75, 0.07-0.18 and 0.56-1.76 mg/g, respectively. Conclusion It was scientific and reasonable to predict Q-Markers of E. acer. The study provides science data for the quality control of E. acer. It also provides a foundation for the development of new resources of breviscapine.

关 键 词：质量标志物 UPLC-Q-TOF-MS 心脑血管疾病 网络药理学 飞蓬 灯盏乙素 灯盏花苷I 绿原酸 异绿原酸A 异绿原酸B 芹菜素 木犀草素 槲皮素 

分 类 号：R286.2[医药卫生—中药学]