^(1)H NMR代谢组学在筛选乳腺浸润性导管癌HER2阳性表达代谢标志物中的应用  

Metabolic biomarkers of breast cancer with HER2 positive expression based on ^(1)HNMR

作　　者：刘影[1] 阳宇[1] 郑细闰 刘红英[1] 吴云山 郑广娟[1] LIU Ying;YANG Yu;ZHENG Xi-run;LIU Hong-ying;WU Yun-shan;ZHENG Guang-juan(Department of Pathology,Guangdong Provincial Hospital of TCM,Guangzhou 510120,Guangdong,China;不详)

机构地区：[1]广东省中医院病理科,广东广州510120 [2]广东省中医药科学院,广东广州510006

出　　处：《广东医学》2022年第7期817-821,共5页Guangdong Medical Journal

基　　金：广东省医学科研基金项目(B2018271);广东省中医药局科研项目(20191137)。

摘　　要：目的分析乳腺浸润性导管癌患者人类表皮生长因子受体2(HER2)基因表达阳性组与阴性组血清代谢组上的差异,为临床HER2表达的准确判读提供参考。方法选取经乳腺穿刺活检被确诊为浸润性乳腺癌的68例女性患者作为研究对象,根据免疫组织化学染色(IHC)结果将其分成两组,包括HER2表达阳性组26例,HER2表达阴性组42例。采用核磁共振波谱仪对两组患者的血清进行图谱采集,应用正交偏最小二乘判别法(OPLS-DA)分析两组间的代谢差异,并使用SPSS软件对代谢物对应图谱的积分面积进行统计分析。结果OPLS-DA分析显示乳腺浸润性导管癌患者HER2表达阳性组与阴性组之间的血清代谢特征存在明显差异。与HER2表达阴性组患者相比,HER2表达阳性组患者血清中亮氨酸/异亮氨酸、丙氨酸、醋酸、谷氨酸盐、牛磺酸的含量显著升高(P<0.05);而3-羟基丁酸、脂质(主要是VLDL)、乳酸的含量显著下降(P<0.05)。结论乳腺浸润性导管癌患者HER2表达阳性与阴性在血清代谢组上差异明显,差异代谢物或可作为潜在的代谢标志物,为临床HER2表达的准确判读提供辅助参考。Objective To analyze the serum metabonomic differences between the human epidermal growth factor receptor 2(HER2)gene expression positive group and negative group of breast invasive ductal carcinoma,and to provide reference for clinic interpretation of HER2.Methods Sixty-eight patients with breast invasive ductal carcinoma,diagnosed by fine needle biopsy were enrolled.They were assigned into 2 groups according to the results of immunohistochemistry(IHC),including HER2 positive group(n=26)and HER2 negative group(n=42).All serum metabolic spectra of the two groups were collected by nuclear magnetic resonance spectrometer,and the metabolic difference between two groups were analyzed by orthogonal signal correction partial least squares discrimination analysis(OPLS-DA).In addition,the spectral integral areas of corresponding metabolites were analyzed by SPSS software.Results OPLS-DA analysis indicated that obvious metabolic difference existed between the positive and negative groups with HER2 expression.Compared to negative group,the levels of leucine/isoleucine,alanine,acetic acid,glutamate and taurine were significantly higher in positive group(P<0.05);while the levels of 3-hydroxybutyric acid,lipid(mainly VLDL)and lactate were significantly lower in positive group(P<0.05).Conclusion Distinct difference exists in serum metabonomic profiles between the positive group and negative group with expression of HER2 in breast invasive ductal carcinoma patients,which may serve as the potential biomarkers and provide auxiliary reference to interpret HER2 expression in clinic.

关 键 词：代谢组学 乳腺浸润性导管癌 HER2 代谢标志物 

分 类 号：R331[医药卫生—人体生理学] R446.112[医药卫生—基础医学]