11β-羟基类固醇脱氢酶Ⅰ型在不同年龄段大鼠眼内的表达与分布  

The Distribution and Expression of 11β-Hydroxysteroid Dehydrogenase Type 1 in Rat Eyes of Different Ages

作　　者：陈薪安 林永 叶菊秀 Xin'an Chen;Yong Lin;Juxiu Ye(School of Basic Medical Science,Wenzhou Medical Universtiy,Wenzhou 325035,China;State Key Laboratory of Ophthalmology and Visual Science,Wenzhou Medical Universtiy,Wenzhou 325027,China)

机构地区：[1]温州医科大学基础医学院,温州325035 [2]温州医科大学附属眼视光医院卫生部视觉科学研究重点实验室,温州325027

出　　处：《中华眼视光学与视觉科学杂志》2022年第8期561-567,共7页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：国家自然科学基金(31301070);浙江省自然科学基金(Y2100924);温州市科技项目(Y2008002)。

摘　　要：目的:观察糖皮质激素代谢酶11β-羟基类固醇脱氢酶Ⅰ型(11β-HSD1)在不同年龄Sprague-Dawlay(SD)大鼠眼内的表达与分布。方法:实验研究。将SD大鼠按年龄分为6组:1~2日龄新生组(PND1)、5~6日龄组(PND5)、10~11日龄组(PND10)、14~15日龄刚开眼组(PND15)、20~21日龄组(PND20)和8~9周龄成年组(M),每组6只。采用免疫组织化学染色法(IHC)检测从新生、开眼到成年等不同发育阶段SD大鼠眼组织内11β-HSD1蛋白的表达和分布情况,并通过Real time RT-PCR和Western blot进一步验证11β-HSD1在不同年龄段大鼠视网膜内的表达差异。组间数据比较采用单因素方差分析或t检验分析。结果:IHC显示11β-HSD1在各年龄组SD大鼠眼角膜、虹膜、视网膜、脉络膜、晶状体、睫状体等组织中均有表达,新生SD大鼠眼组织中的11β-HSD1阳性细胞数更多,染色更明显。Real time RT-PCR结果显示,角膜内11β-HSD1 mRNA相对表达量在SD大鼠出生后10 d达到高峰,SD大鼠开眼后开始逐渐下降,差异有统计学意义(F=8.40,P<0.001)。开眼前SD大鼠视网膜内的11β-HSD1 mRNA相对表达量高于开眼后和成年,差异有统计学意义(F=83.50,P<0.001)。Western bolt进一步证实,新生大鼠视网膜内的11β-HSD1蛋白表达量高于刚开眼大鼠和成年大鼠,差异有统计学意义(t=4.76,P=0.009;t=4.24,P=0.013)。结论:11β-HSD1在SD大鼠眼组织内广泛分布,其表达量随年龄而变化,开眼前SD大鼠眼组织尚未分化发育成熟,其表达量较高,提示11β-HSD1可能与SD大鼠眼组织的分化、发育和成熟有关。Objective:To observe the distribution and expressive changes of 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1)in different age’s rat eyes.Methods:In this experimental research,according to age,SD rats were divided into 6 groups with 6 subjects in each:postnatal days 1-2(PND1)group,postnatal days 5-6(PND5)group,postnatal days 10-11(PND10)group,postnatal days 14-15(PND15)group,postnatal days 20-21(PND20)group,and 8-9 weeks mature adults rats(M)group.Immunohistochemistry(IHC)assay was used to detect the distribution of 11β-HSD1 protein in the eyes of SD rats at different developmental stages from neonatal,postnatal eyelid opening to mature adult,and the expressions of 11β-HSD1 mRNA and protein levels in the retina or cornea were further verified by real time RT-PCR and Western blot.The data were analyzed by one-way ANOVA or independent sample t-test.Results:11β-HSD1 protein was widely expressed in such as cornea,iris,retina,choroid,lens,ciliary body of the different age’s rat eyes by IHC staining,and there was more 11β-HSD1 positive cells in neonatal rat eyes.Real time RT-PCR showed that the relative expression of mRNA of 11β-HSD1 peaked at postnatal days 10 in the cornea and decrease gradually after eyelid opening of SD rats(F=8.40,P<0.001);the relative expression of 11β-HSD1 mRNA in the retina of SD rats before eyelid opening was significantly higher than that of eyelid opening rats and adult rats(F=83.50,P<0.001).It was confirmed the relative expression of 11β-HSD1 protein was significantly higher in the retina of neonatal rats than that of eyelid opening rats and mature adult rats by western blot(t=4.76,P=0.009;t=4.24,P=0.013).Conclusion:11β-HSD1 is widely distributed in SD rat eyes of different ages.The level of 11β-HSD1 expression varies in different age groups.It tends to be higher in eyes of SD rat before eyelid opening.The study suggests that 11β-HSD1 may play an important role in the differentiation,development and maturation of the SD rat eyes.

关 键 词：11β-羟基类固醇脱氢酶Ⅰ型 糖皮质激素 眼组织 表达 发育 

分 类 号：R339.14[医药卫生—人体生理学]