乌苏里拟鲿igf基因克隆及其与生长性状关联分析  

Cloning and Association Analysis with Growth Traits of igf Gene in Ussuri Catfish Pseudobagrus ussuriensis

作　　者：魏梦雅 王思梦 宁静 范帆 朱传坤 WEI Mengya;WANG Simeng;NING Jing;FAN Fan;ZHU Chuankun(Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection,Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms,School of Life Science,Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake,Huaiyin Normal University,Huaian 223300,China)

机构地区：[1]淮阴师范学院生命科学学院,江苏省环洪泽湖生态农业生物技术重点实验室,江苏省特色水产繁育工程实验室,江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300

出　　处：《水产科学》2022年第5期738-748,共11页Fisheries Science

基　　金：国家自然科学基金资助项目(31602146);江苏省高等学校大学生创新创业训练计划项目(201810323049X);淮阴师范学院博士科研启动项目(31ZCK00)。

摘　　要：为探究胰岛素样生长因子基因igf与生长性状间的关系,以近缘物种igf基因为参考,克隆乌苏里拟鲿igf1和igf2基因序列,并利用关联分析法分析其多态位点与生长性状的关系。研究结果显示:获得igf1基因全长cDNA 1241 bp,其中开放阅读框(ORF)长477 bp,编码158个氨基酸,igf1基因部分DNA序列10 922 bp, igf1基因由5个外显子和4个内含子组成;获得igf2基因全长cDNA 2008 bp, ORF长741 bp,编码246个氨基酸,igf2基因DNA部分序列3700 bp, igf2基因由4个外显子和3个内含子组成。两基因编码的IGF1和IGF2氨基酸序列均与黄颡鱼相应序列的一致性最高(96.9%和96.7%)。在igf1基因中共检测到5个单核苷酸多态性(SNP)位点,其中1个位于外显子,4个位于内含子,并检测出7个微卫星位点;在igf2基因中共检测到5个SNP位点,其中1个位于外显子、4个位于内含子,并检测出5个微卫星位点。挑选igf1和igf2基因中位于外显子的SNP位点与5个生长性状进行了关联分析,但未检测到显著关联性。本研究结果可为乌苏里拟鲿igf基因的深入研究提供参考。In order to understand the relationship between insulin-like growth factor(igf) genes and growth traits, sequences of igf1 and igf2 genes were cloned for Ussuri catfish Pseudobagrus ussuriensis through referring to igf sequences of its close-related fishes, and relationships between polymorphic sites of igf and growth traits were analyzed by association analysis. The cDNA length of the igf1 gene was 1241 bp, containing an open reading frame(ORF) of 477 bp coding a peptide with 158 amino acids(aa), and the DNA sequence of the igf1 gene with a length of 10 922 bp containing 5 exons and 4 introns was also obtained. The total cDNA length of the igf2 gene was 2008 bp with an ORF of 741 bp coding a peptide consisting 246 aa, and a 3700 bp DNA segment containing 5 exons and 4 introns was cloned. The aa sequences of the two peptides IGF1 and IGF2 of Ussuri catfish had the highest identities with those of yellow catfish Pelteobagrus fulvidraco(96.9% and 96.7%). A total of five simple sequence polymorphism sites(SNPs) were identified in the igf1 gene with 1 in exon and 4 in introns, and 7 microsatellites were also found. The number of identified SNPs in the igf2 gene was also 5 with 1 in exon and 4 in introns, and 5 microsatellites were identified. The 2 SNPs in exons of igf1 and igf2 genes were selected and their associations with 5 growth traits were also analyzed, but no significant associations were detected. Nevertheless, the finding in this study will provide useful references for further studies of igf genes in Ussuri catfish.

关 键 词：乌苏里拟鲿 igf1基因 IGF2基因 单核苷酸多态性 生长 关联分析 

分 类 号：S917.4[农业科学—水产科学]