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作 者:陶莎 张效平 商宝娣 李小义 姚俊杰[2] TAO Sha;ZHANG Xiaoping;SHANG Baodi;LI Xiaoyi;YAO Junjie(Guizhou Fisheries Research Institute,Guiyang 550025,China;Center of Fisheries Resources and Environment,Guizhou University,Guiyang 550025,China;Guiyang Special Aquatic Engineering Technology Center,Guiyang 550025,China)
机构地区:[1]贵州省农业科学院水产研究所,贵州贵阳550025 [2]贵州大学渔业资源与环境研究中心,贵州贵阳550025 [3]贵州省特种水产工程技术中心,贵州贵阳550025
出 处:《水产科学》2022年第5期883-890,共8页Fisheries Science
基 金:国家自然科学基金地区科学基金资助项目(31560729);贵州省农业公关项目(黔科合NY[2015]3003-1号);国家特色淡水鱼产业技术体系贵阳综合试验站项目(CARS-46);贵州省特色水产产业技术体系(GZCYTX2019-01102);贵州省农业科学院青年基金资助项目(黔农科院青年基金[2018]51号)。
摘 要:为有效控制因嗜水气单胞菌感染斑点叉尾(鱼回)造成的经济损失,通过水煎法获得金银花、夏枯草、石榴皮等41味中草药的提取物,测定中草药及其复方对嗜水气单胞菌的体外抑杀效果,包括抑菌圈直径、最小抑菌质量浓度及最小杀菌质量浓度3项指标,综合筛选出体外抑菌效果较好的单方及复方中草药。试验结果表明,14种单方中草药具有抑菌圈,最小抑菌质量浓度最低为黄连的7.81 mg/mL,综合最小杀菌质量浓度结果,筛选出抑菌效果较好的6种单方中草药,即石榴皮、诃子、紫花地丁、苏木、黄连、乌梅;药物联用结果显示,6种单方之间未出现拮抗的比例为93.3%,联用效果较好的比例为80%;根据正交表设计的25组复方结果显示,V(石榴皮)∶V(诃子)∶V(紫花地丁)∶V(苏木)∶V(黄连)∶V(乌梅)=2∶5∶1∶2∶3∶4(M-10复方组)的综合抑菌效果最好。本结果可为中草药治疗嗜水气单胞菌细菌性疾病的应用提供理论基础。To effectively control the economic loss caused by infection of Aeromonas hydrophila from channel catfish Ictalurus punctatus, 41 kinds of extracts of Chinese herbal medicine were obtained by decoction, such as Flos Lonicerae, Spica Prunellae and Pericarpium Granati. The diameter of inhibition circle, the minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of 41 kinds of Chinese herbal medicine and their compound to A. hydrophila were detected respectively. The results showed that 14 of 41 Chinese herbs had bacteriostasis circle, with the minimal MIC in Rhizoma Coptidis at concentration of 7.81 mg/mL, comprehensive diameter of inhibition circle, MIC and MBC, comprehensive screening of 6 Chinese herbs with better bacteriostatic effect: Pericarpium Granati, Fructus Chebulae, Herba Violae, Lignum Sappan, Rhizoma Coptidis and Fructus Mume. Drug combination results showed that there was no antagonism of 93.3% between the 6 herbs, with positive effect of 80%. The results of 25 groups according to preparation tables of mixed Chinese herbal medicine showed that M-10 compound group with V(Pericarpium Granati)∶V(Fructus Chebulae)∶V(Herba Violae)∶V(Lignum Sappan)∶V(Rhizoma Coptidis)∶V(Fructus Mume) = 2∶5∶1∶2∶3∶4 had the best comprehensive bacteriostatic effect. The findings provide a theoretical basis for the application of Chinese herbal medicine in bacterial diseases of A. hydrophila.
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