C57BL/6小鼠Lewis皮下移植瘤肺转移灶的病理及超微结构观察  

Pathological and ultrastructural observations of lung metastases from Lewis subcutaneous grafts in C57BL/6 mice

作　　者：张娜[1] 刘学芳[2] 王自闯[3] 冯素香[2] 张鹏飞[2] ZHANG Na;LIU Xuefang;WANG Zichuang;FENG Suxiang;ZHANG Pengfei(College of Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Academy of Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046;the Second Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou 450046)

机构地区：[1]河南中医药大学医学院,郑州450046 [2]河南中医药大学中医药科学院,郑州450046 [3]河南中医药大学第二附属医院,郑州450046

出　　处：《中国实验动物学报》2022年第4期476-481,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(82003986)。

摘　　要：目的构建C57BL/6小鼠Lewis皮下移植瘤肺转移模型,观察肺转移灶病理以及超微结构变化。方法C57BL/6小鼠右前肢腋窝皮下接种Lewis细胞,定期测量肿瘤体积,绘制生存曲线,对存活期35 d以上的小鼠进行解剖学观察,HE染色检测肺组织病理变化,透射电镜观察肺组织的超微结构。结果接种14 d小鼠右前肢腋窝皮下成瘤率为100%,22 d小鼠开始出现死亡,平均生存周期(33.00±6.98)d;解剖学可见肺表面出现典型转移灶,外观呈圆形半透明状,周围有出血;HE染色显示肺转移灶染色深,外观近似圆形,与周围组织界限明显,肿瘤细胞排列紧密,内部可见丰富的毛细血管;透射电镜下可见肺转移灶内肿瘤细胞具有显著的异型性,出现巨核、双核和奇异型核,胞质内含有大量的糖原颗粒和线粒体数量,肿瘤细胞间可见细胞连接。结论延长存活时间可使皮下移植瘤小鼠形成典型的肺转移灶,肺转移灶内病理学和超微结构变化符合恶行肿瘤的病变特点。Objective To establish a lung metastasis model based on Lewis subcutaneously-implanted tumors in C57BL/6 mice,and observe the pathological and ultrastructural changes in lung metastasis.Methods C57BL/6 mice were subcutaneously implanted with Lewis cells in the armpit of the right forelimb.The tumor volume was measured regularly and a survival curve was generated.Anatomical observations were performed on mice whose survival time was more than 35 days,hematoxylin-eosin(HE)staining was used to detect pathological changes in the lung tissue,and the ultrastructure of the lung tissue was observed by transmission electron microscopy.Results The subcutaneous tumor formation rate of the right forelimb axillary was 100%at 14 days after inoculation,and mortality occurred from 22 days,with a mean life span for the mice of(33.00±6.98)days.Anatomically,typical metastases were observed on the lung surface,which had a round translucent appearance and surrounding hemorrhage.HE showed intense staining of the lung metastases,which were nearly round in appearance,with obvious boundaries with the surrounding tissue.The tumor cells were closely arranged and contained abundant capillaries.Under transmission electron microscopy,the tumor cells in the lung metastases showed significant atypia,including meganucleus,dicaryon,and singular nuclei.The cytoplasm of the cells contained a large number of glycogen particles and mitochondria,and cell junctions were evident between tumor cells.Conclusions Prolongation of the survival time resulted in the formation of typical lung metastases in mice with a subcutaneous tumor.The pathological and ultrastructural changes in lung metastases were consistent with the characteristics of malignant tumors.

关 键 词：LEWIS肺癌 肺转移 病理 超微结构 细胞连接 

分 类 号：Q95-33[生物学—动物学]