山羊痘病毒在不同日龄山羊睾丸细胞中的生长特性研究  

Proliferation Characteristics of Goat Pox Virus in Goat Testicular Cells of Different Ages

作　　者：牟豪 余远迪 许国洋 杨柳[1,2] MU Hao;YU Yuandi;XU Guoyang;YANG Liu(Chongqing Academy of Animal Sciences,Chongqing 402460,China;Chongqing Veterinary Biological Products Engineering Technology Research Center,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]重庆市兽用生物制品工程技术研究中心,重庆402460

出　　处：《云南农业大学学报（自然科学版）》2022年第5期799-803,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：重庆荣昌农牧高新技术产业研发专项(cstc2019ngzx0012);重庆市技术创新与应用发展专项(cstc2019jscx-msX0325);国家动物疫病长期性观测监测项目(18501)。

摘　　要：【目的】明确山羊痘病毒(GTPV)AV41弱毒疫苗株在不同日龄山羊原代睾丸细胞中的增殖效率,为选择病毒或疫苗株的培养细胞提供数据支撑。【方法】通过传统方式对7日龄和60日龄山羊睾丸细胞分离培养,利用长时间动态细胞成像及数据分析系统比较2个日龄细胞的生长效率;接种GTPV,观察2个日龄细胞在感染病毒后的形态特征;通过间接免疫荧光和荧光定量PCR比较GTPV的增殖效率。【结果】7日龄和60日龄山羊睾丸细胞的形态特征和生长速率无明显差异;接种GTPV后,2个日龄的原代山羊睾丸细胞均能产生细胞病变,但是出现细胞病变的时间以及病变特征存在差别。间接免疫荧光和荧光定量PCR结果表明:GPTV在60日龄山羊睾丸细胞中的增殖效率高于7日龄。【结论】GTPV在7日龄和60日龄山羊原代睾丸细胞中均能发生病毒增殖,且2种细胞均能发生明显的细胞病变,但60日龄细胞的增殖效率高于7日龄细胞。研究结果对采用山羊睾丸细胞培养GTPV弱毒株有一定的指导作用。[Purpose]To determine the proliferation efficiency of goat pox virus(GTPV)AV41 attenuated vaccine strain in primary testicular cells of goats at different ages,and to provide datas to support the selection of cultured cells for virus or vaccine strains.[Methods]Two ages of cells(7-day-old and 60-day-old goat testicular cells)were isolated and cultured in traditional way.The growth efficiency of the two ages of cells was compared by the long-term dynamic cell imaging and data analysis system,and then GTPV was inoculated to observe the morphological characteristics.The propagation efficiency of GTPV was compared by indirect immunofluorescence(IFA)and fluorescent quantitative PCR(qPCR).[Results]There was no obvious differences in morphological characteristics and growth rate between the two ages of cells.After GTPV inoculation,the two ages of primary goat testicular cells could produce cytopathic effect(CPE),but there were differences in the occurrence time and CPE characteristics.IFA and qPCR results showed that the propagation effi-ciency of GTPV in 60-day-old goat testicular cells was higher than that in 7-day-old goat testicular cells.[Conclusion]Both 7-day-old and 60-day-old goat primary testicular cells can develop obvi-ous CPE after inoculation with GTPV.GTPV can proliferate in both cells,but the propagation effi-ciency is different.These results may be useful for the culture of GTPV attenuated strain with goat testis cells.

关 键 词：山羊原代睾丸细胞 山羊痘病毒 细胞病变 增殖效率 

分 类 号：S858.275.3[农业科学—临床兽医学]