黄芪超微粉抗急性肝损伤、抗疲劳作用及黄芪甲苷含量的变化  

Anti Acute Liver Injury and Anti Fatigue Effects of Huangqi Ultrafine Powder and Changes of Astragaloside IV Content

作　　者：柴梦音[1,2] 寇卜心 豆双双[1,2] 刘晓霓 CHAI Mengyin;KOU Buxin;DOU Shuangshuang;LIU Xiaoni(Beijing You an Hospital Affiliated to Capital Medical University,Beijing 100069,China;Beijing Institute of Liver Disease,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]北京肝病研究所,北京100069

出　　处：《现代中医药》2022年第5期26-32,共7页Modern Chinese Medicine

基　　金：北京市属医学科研院所公益发展改革试点项目(京医研2019-6);北京市卫生系统高层次卫生技术人才培养计划(2015-3-101)。

摘　　要：目的观察黄芪超微粉的抗急性肝损伤、抗疲劳作用及黄芪甲苷含量的变化,为黄芪超微粉的应用提供科学依据。方法采用低温超微粉碎技术将黄芪饮片粉碎成超微粉,利用显微镜和激光粒度仪观察其显微表征和粒度表征,采用高效液相法检测黄芪饮片和黄芪超微粉中黄芪甲苷的含量。取昆明种小鼠,随机分为对照组(Con)、模型组(M)、黄芪饮片组(HQY,10 g·kg^(-1))、黄芪超微粉高剂量组(HQFH,10 g·kg^(-1))、黄芪超微粉中剂量组(HQFM,2 g·kg^(-1))、黄芪超微粉低剂量组(HQFL,0.4 g·kg^(-1))。除对照组和模型组,各药物组提前给药7 d,对照组给予相同体积生理盐水。末次给药后,模型组和各药物组腹腔注射0.1%四氯化碳(CCl 4)橄榄油10 mL·kg^(-1),18 h后眼眶采血,分离血浆,检测AST、ALT;取部分肝组织观察组织病理学改变。另取昆明种小鼠,分组与给药同前,末次给药后,按照体重10%负重,将小鼠置入水中游泳(25℃),并开始计时。当小鼠沉入水中不再浮出水面5 s,停止计时,计算小鼠游泳时间,自眼眶取血,检测血中乳酸含量。对照组不进行游泳,实验终点取血检测乳酸含量。结果显微镜和粒度仪显示黄芪饮片被粉碎成大小不等的细小颗粒,粒径分布范围为0.45~709μm,小于80μm的颗粒累积大于80%。黄芪超微粉(0.4~10 g·kg^(-1))可以减少0.1%CCl_(4)所致的肝坏死和AST,ALT的升高(P<0.05)。黄芪超微粉(0.4~10 g·kg^(-1))可以明显提高小鼠游泳时间(P<0.05),减少小鼠血中乳酸含量(P<0.05)。0.4 g·kg^(-1)黄芪超微粉的保肝作用和抗疲劳作用与10 g·kg^(-1)的黄芪饮片相当。高效液相显示黄芪超微粉的黄芪甲苷含量明显升高。结论与黄芪饮片相比,黄芪超微粉具有更好的保肝耐疲劳的作用,可能与黄芪超微粉中黄芪甲苷成分含量增高有关。Objective To observe the anti acute liver injury and anti fatigue effects of Huangqi ultrafine powder and the changes of astragaloside IV content,so as to provide scientific basis for the application of Huangqi ultrafine powder.Methods Astragalus pieces were crushed into ultra-fine powder by low-temperature ultra-fine pulverization technology.The microscopic characterization and particle size characterization were observed by microscope and laser particle sizer.The content of Astragaloside IV in Astragalus pieces and Huangqi ultra-fine powder was detected by HPLC.Kunming mice were randomly divided into control group(Con),model group(M),Huangqi decoction group(HQY,10 g·kg^(-1)),Huangqi ultra-fine powder high-dose group(HQFH,10 g·kg^(-1)),Huangqi ultra-fine powder medium dose group(HQFM,2 g·kg^(-1)),Huangqi ultra-fine powder low-dose group(HQFL,0.4 g·kg^(-1)).Except for the control group and the model group,each drug group was given 7 days in advance,and the control group was given the same volume of normal saline.After the last administration,the model group and each drug group were intraperitoneally injected with 10 mL·kg^(-1) of 0.1%carbon tetrachloride(CCl_(4))olive oil.After 18 hours,orbital blood was collected,plasma was separated,and AST and ALT were detected;some liver tissues were taken to observe histopathological changes.Another Kunming mice were taken,and the grouping was the same as that of the administration.After the last administration,the mice were put into water to swim(25℃)according to 10%weight,and began timing.When the mouse sank into the water and no longer surfaced for 5 seconds,stop timing,calculate the swimming time of the mouse,take blood from the orbit,and detect the lactic acid content in the blood.The control group did not swim,and the lactic acid content was detected by blood sampling at the end of the experiment.Results Microscope and particle size analyzer showed that Astragalus pieces were crushed into small particles of different sizes,with particle size distribution ranging

关 键 词：黄芪 急性肝损伤 游泳实验 中药超微粉 黄芪甲苷 

分 类 号：R283[医药卫生—中药学]