Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth,development,and symbiotic nodule development  

作　　者：Shengcai Chen Yaqi Peng Qi Lv Jing Liu Zhihua Wu Haijiao Wang Xuelu Wang 

机构地区：[1]College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Key Laboratory of Plant Stress Biology,Henan University,Kaifeng 475001,China [3]Sanya Institute of Henan University,Sanya 572025,China [4]Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China

出　　处：《aBIOTECH》2022年第2期99-109,共11页生物技术通报（英文版）

基　　金：supported by National Natural Science Foundationof China(Grant No.31870257 and U21A20181 to X.W,Grant no.32170728 to H.W.);the National Key Research,and Development,Program(Grant No.2018YFE0112100 to X.W.);Excellent Youth Foundation of Henan Province(Grant No.222300420025 to H.W.);the 111 Project of China(Grant No.D16014).

摘　　要：Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at multiple growth and development stages and during nodule development.Here,we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28(RPS28)and EUKARYOTIC TRANSLATION INITIATION FACTOR 1(EIF1)are ideal for high expression of transgene.Through bioinformatic analysis,we determined that RPS28 and EIF1 were highly expressed during soybean growth and development,nodule development,and various biotic and abiotic stresses.Fusion of both RPS28 and EIF1 promoters,with or without their first intron,with the reporter geneβ-GLUCURONIDASE(uidA)in transgenic soybean,resulted in high GUS activity in seedlings,seeds,and nodules.Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression,comparable to the soybean Ubiquitin(GmUbi)promoter.RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana.Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean,as well as in a wide variety of other plant species.

关 键 词：PROMOTER Soybean(Glycine max) Ribosomal protein S28 Translation initiation factor EIF1 Constitutive expression 

分 类 号：Q94[生物学—植物学]