Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9  

作　　者：Zhihui Zhang Jie Wang Huaqin Kuang Zhihong Hou Pingping Gong Mengyan Bai Shaodong Zhou Xiaolei Yao Shikui Song Long Yan Yuefeng Guan 

机构地区：[1]College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [2]FAFU-UCR Joint Center for Horticultural Biology and Metabolomics,Fujan Agriculture and Forestry University,Fuzhou 350002,Fujian,China [3]College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China [4]College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujan,China [5]The Key Laboratory of Crop Genetics and Breeding of Hebei,Institute of Cereal and oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050035,China

出　　处：《aBIOTECH》2022年第2期110-114,共5页生物技术通报（英文版）

基　　金：supported by Innovative Research Groups of the Natural Science Foundation of Hebei province(C2020301020).

摘　　要：Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding.Nevertheless,a significant portion of soybean cultivars are still pod shattering-susceptible,limiting their regional and climatic adaptabilities.Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar,Huachun6(HC6),and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1(PDH1)gene,which exists in a significant portion of soybean cultivars.We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPR-associated protein 9(Cas9).In T2 progenies,several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits.The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma.Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars.

关 键 词：Genome editing Pod shattering SOYBEAN Precision breeding Marker assisted selection CRISPR/Cas9 

分 类 号：Q78[生物学—分子生物学]