Study on the mechanism of moxibustion for rheumatoid arthritis based on liquid chromatography-mass spectrometry  

基于液相色谱-质谱法的艾灸治疗类风湿关节炎机制研究

作　　者：PANG Xiangtian LENG Yufei YAO Yao WANG Danwen LI Cheng XU Xiao SUN Zhiling 庞祥天;冷雨飞;姚瑶;王丹文;李程;徐骁;孙志岭(School of Nursing,Nanjing University of Chinese Medicine,Nanjing 210023,China;Basic Medicine,Nanjing Vocational Health College,Nanjing 210038,China;School of Nursing,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]School of Nursing,Nanjing University of Chinese Medicine,Nanjing 210023,China [2]Basic Medicine,Nanjing Vocational Health College,Nanjing 210038,China [3]School of Nursing,Zhejiang Chinese Medical University,Hangzhou 310053,China

出　　处：《Journal of Acupuncture and Tuina Science》2022年第4期247-256,共10页针灸推拿医学（英文版）

基　　金：国家自然科学基金项目,No.81774383,No.81904274;江苏省高校护理优势学科建设项目,No.2019YSHL008,No.2019YSHL021;江苏省研究生科研创新计划项目,No.KYCX21_1802.

摘　　要：Objective To explore the mechanism of moxibustion for rheumatoid arthritis(RA)by observing the metabolite changes in urine using liquid chromatography-mass spectrometry(LC-MS)-based metabolomic analysis.Methods Twenty-four rats were randomly divided into a control group,a model group,and a moxibustion group.Rats in the model and moxibustion groups were established as collagen-induced arthritis(CIA)models.The control and model groups did not receive any intervention;rats in the moxibustion group received moxibustion at Shenshu(BL23)and Zusanli(ST36).After three weeks of intervention,ankle joint,serum,and urine samples were collected for pathological examinations and metabolomic tests.Results After moxibustion treatment,the CIA rats showed increased body mass,reduced swelling of the hind paws and arthritis score,decreased serum cytokine levels,and improved histopathological evaluation of the ankle joint.Twenty-four significantly altered metabolites were found,mainly involved in alanine metabolism,taurine and hypotaurine metabolism,tricarboxylic acid cycle,phenylalanine metabolism,tyrosine metabolism,and primary bile acid biosynthesis.These metabolites may serve as potential biomarkers for RA.Conclusion Moxibustion can effectively resist inflammation in CIA rats.The potential biomarkers and the abnormal metabolic pathways of RA can be identified by LC-MS-based metabolomics.Metabolomics may be an effective way to explain the mechanism of moxibustion in treating RA.目的通过代谢组学液相色谱-质谱法(LC-MS)比较尿液代谢物的变化,以探索艾灸治疗类风湿关节炎(RA)的机制。方法将24只大鼠随机分为对照组,模型组和艾灸组。模型组和艾灸组大鼠建立胶原性关节炎(CIA)模型。对照组和模型组不干预;艾灸组大鼠接受艾灸肾俞和足三里治疗。干预3周后,收集踝关节,血清和尿液标本进行病理学和代谢组学检测。结果艾灸治疗后,CIA大鼠体质量增加,后足肿胀度减轻,关节炎评分及血清细胞因子水平降低,踝关节的组织病理形态改善。共发现24种显著变化的代谢产物,主要涉及丙氨酸代谢,牛磺酸和次牛磺酸代谢,三羧酸循环,苯丙氨酸代谢,酪氨酸代谢和初级胆汁酸的生物合成。这些代谢物可作为RA的潜在生物标记。结论艾灸治疗可以有效抵抗CIA大鼠的炎症。RA的潜在生物标志物和异常代谢途径可通过代谢组学LC-MS被确定。代谢组学可能是解释艾灸治疗RA机制的有效途径。

关 键 词：Moxibustion Therapy Arthritis Rheumatoid Arthritis Experimental Mass Spectrometry Metabolomics Rats 

分 类 号：R2-03[医药卫生—中医学]