Protective Effect of Fresh/Dry Dandelion Extracts on APAP-Overdose-Induced Acute Liver Injury  

作　　者：ZHENG Yao LEI Lei LIANG Shuai AI Jiao DENG Xin LI Yan-qiu ZHANG Tian-pei PU Shi-biao REN Yong-shen 

机构地区：[1]School of Pharmaceutical Science,South-Central University for Nationalities,Wuhan,430074,China [2]College of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming,650500,China

出　　处：《Chinese Journal of Integrative Medicine》2022年第8期683-692,共10页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81773893,81760812);National Major Scientific and Technological Special Project for"Significant New Drugs Development"(No.2017ZX09301060-001);Jiangxi Province"Thousand Talents Plan"of Scientific and Technological Innovation(No.JXSQ2019201105);Yunnan Province"Ten Thousand Talents"Special Support Plan(No.YNWRQNBJ-2018-190)。

摘　　要：Objecitvie:To compare the liver protective activity of fresh/dried dandelion extracts against acetaminophen(APAP)-induced hepatotoxicity.Methods:Totally 90 Kunming mice were randomly divided into10 groups according to body weight(9 mice for each group).The mice in the normal control and model(vehicle control)groups were administered sodium carboxymethyl cellulose(CMC-Na,0.5%)only.Administration groups were pretreated with high and low-dose dry dandelion extract(1,000 or 500 g fresh herb dried and then decocted into 120 mL solution,DDE-H and DDE-L);low-,medium-and high-dose dandelion juice(250,500,1,000 g/120 mL,DJ-L,DJ-M,and DJ-H);fresh dandelions evaporation juice water(120 mL,DEJW);dry dandelion extract dissolved by pure water(1 kg/120 mL,DDED-PW);dry dandelion extract dissolved by DEJW(120 g/120 mL,DDED-DEJW)by oral gavage for 7 days at the dosage of 0.5 mL solution/10 g body weight;after that,except normal control group,all other groups were intraperitonealy injected with 350 mg/kg APAP to induce liver injury.Twenty hours after APAP administration,serum and liver tissue were collected and serum alanine aminotransferase(AST),aspartate transaminase(ALT),alkaline phosphatase(AKP),malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD)activities were quantified by biochemical kits;tumor necrosis factor(TNF-α),interleukin(IL)-2,and IL-1β contents in liver tissue were determined by enzyme linked immunosorbent assay kits.Histopathological changes in liver tissues were observed by hematoxylin and eosin staining;TUNEL Assay and Hoechst 33258 staining were applied for cell apoptosis evaluation.The expressions of heme oxygenase-1(HO-1),nuclear factor erythroid-2-related factor 2(Nrf-2),caspase-9,B-cell leukemia/lymphoma 2(Bcl-2),Bax and p-JNK were determined by Western blot analysis.Results:Pretreatment with fresh dandelion juice(FDJ,including DJ-L,DJ-M,DJ-H,DEJW and DDED-DEJW)significantly decreased the levels of serum ALT,AST,AKP,TNF-α and IL-1β compared with vehicle control group(P<0.05 or P<0.01).Additio

关 键 词：fresh dandelion juice acetaminophen-induced liver injury oxidative stress anti-inflammation ANTI-APOPTOSIS 

分 类 号：R285.5[医药卫生—中药学]