补肺益肾组分方Ⅲ联合运动康复对慢性阻塞性肺疾病大鼠的影响  

Effect of Effective-component Compatibility of Bufei Yishen FormulaⅢCombined with Exercise Rehabilitation in Chronic Obstructive Pulmonary Disease Rats

作　　者：李建生[1] 朱丽华 田燕歌[1,2] 刘帅[1] 金凡力 陈锴 张蓝煕 蔡泽慧 李康沉 李敏艳 LI Jian-sheng;ZHU Li-hua;TIAN Yan-ge;LIU Shuai;JIN Fan-li;CHEN Kai;ZHANG Lan-xi;CAI Ze-hui;LI Kang-chen;LI Min-yan(Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan&Education Ministry of P.R.,Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine,Zhengzhou 450046;Academy of Chinese Medicine Sciences,Henan University of Chinese Medicine,Zhengzhou 450046)

机构地区：[1]河南中医药大学,呼吸疾病中医药防治省部共建协同创新中心,河南省中医药防治呼吸病重点实验室,郑州450046 [2]河南中医药大学中医药科学院,郑州450046

出　　处：《中国中西医结合杂志》2022年第8期987-995,共9页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.81973822);河南省重点研发与推广专项(科技攻关)(No.192102310427)。

摘　　要：目的基于起效时间和效应探讨补肺益肾组分方Ⅲ联合运动康复对慢性阻塞性肺疾病(COPD)大鼠的影响。方法240只大鼠随机分为空白组(Control)、模型组(Model)、补肺益肾组分方Ⅲ组(ECC-BYFⅢ)、运动康复组(ER)、补肺益肾联合运动康复组(ECC-BYFⅢ+ER)、氨茶碱组(APL),每组40只。1~8周采用香烟联合细菌感染法复制COPD大鼠模型,9~16周给予相应干预处理,分别于第10、12、16、20、24周检测肺功能、炎症因子及肺组织病理等。结果与Control组比较,Model组肺功能下降、肺组织病理损伤、炎症反应明显(P<0.05,P<0.01)。与Model组比较,ECC-BYFⅢ组第10~24周IL-6表达及支气管壁厚度(Wt)降低,第12~24周IL-10、单位面积平均肺泡数(MAN)升高,第16~24周肺功能最大呼气中段流速(MMEF)、CD4^(+)/CD8^(+)升高(P<0.05,P<0.01);ER组10~24周IL-6、Wt降低,IL-10升高,第16~24周平均肺泡截距(MLI)降低、MAN升高(P<0.05,P<0.01);ECC-BYFⅢ+ER组第10~24周MLI、Wt、IL-6、TNF-α均降低,IL-10、CD4^(+)升高,第12~24周呼气中期流速(EF50)、MAN升高,FRC降低,第16~24周TV、CD4^(+)/CD8^(+)升高(P<0.05,P<0.01);APL组第10-24周Wt、IL-6、TNF-α降低,12~24周MLI降低、MMEF升高,第16周CD4^(+)/CD8^(+)升高(P<0.05,P<0.01)。与APL组比较,ECC-BYFⅢ+ER组第16周TNF-α降低,ECC-BYFⅢ+ER组第12、20、24周IL-10升高,第24周IL-6降低(P<0.05,P<0.01)。与ER组比较,ECC-BYFⅢ和ECC-BYFⅢ+ER组第24周IL-6降低,ECC-BYFⅢ+ER组12~20周IL-10升高(P<0.05,P<0.01)。结论各方案干预COPD大鼠均具有较好的效应,其中联合干预在改善肺功能、病理损伤及调节炎症免疫方面均起效早、持续时间长,且改善炎症优于运动康复,改善炎症免疫优于氨茶碱。Objective To evaluate the effect of effective-component compatibility of Bufei Yishen FormulaⅢ(ECC-BYFⅢ)combined with exercise rehabilitation(ER)in treatment of chronic obstructive pulmonry disease(COPD)rats based on the onset time and long-term effects.Methods A total of 240 rats were randomly divided into control,model,ECC-BYFⅢ,ER,ECC-BYFⅢ^(+)ER and Aminophylline(APL)groups,40 rats in each group.From week 1 to 8,the COPD rat model was established by cigarette exposure combined with repeated klebsiella pneumoniae infection method,and the corresponding intervention treatment was given at week 9 to 16.Pulmonary function,inflammatory factors,and lung tissue pathology were measured at week 10,12,16,20,24.Results Compared with the control group,the rats in model group showed decreased lung function,damaged lung tissue and aggravated inflammatory response(P<0.05,P<0.01).Compared with the model group,the level of IL-6 and bronchial wall thickness(Wt)decreased from week 10 to 24,the level of IL-10 and mean alveolar numbers(MAN)increased from week 12 to 24,and maximum midexpiratory flow(MMEF)and CD4^(+)/CD8^(+)increased from week 16 to 24 in ECC-BYFⅢgroup(P<0.05,P<0.01);the level of IL-6 and Wt decreased,while the level of IL-10 increased from week 10 to 24,MLI decreased and MAN increased from week 16 to 24 in ER group(P<0.05,P<0.01);MLI,Wt,the levels of IL-6 and TNF-αdecreased,while the level of IL-10 and CD4^(+)increased from week 10 to 24,50%expiratory flow(EF50)and MAN increased,while FRC decreased from week 12 to 24,TV and CD4^(+)/CD8^(+)increased from week 16 to 24in ECC-BYFⅢ^(+)ER group(P<0.05,P<0.01);the Wt,the levels of IL-6 and TNF-αdecreased at week 10 to 24,MLI decreased and MMEF increased at week 12 to 24,CD4^(+)/CD8^(+)increased at week 16 in APL group(P<0.05,P<0.01).Compared with APL group,the level of TNF-αdecreased in ECC-BYFⅢand ECCBYFⅢ^(+)ER groups at week 16,while the level of IL-10 increased in ECC-BYFⅢ^(+)ER group at week 12,20,24,and the level of IL-6 decreased at week 24(P<0.0

关 键 词：慢性阻塞性肺疾病 补肺益肾方 运动康复 疗效特点 中药 

分 类 号：R563.9[医药卫生—呼吸系统]