纳米金比色法检测乙肝病毒核酸综合性实验  

A Comprehensive Experiment of Gold Nanoparticles-based Colorimetry for Hepatitis B Virus Nucleic Acid Detection

作　　者：温聪颖[1] 李想 张瑞巧 李怡敏 周亭[1] 曾景斌[1] WEN Congying;LI Xiang;ZHANG Ruiqiao;LI Yimin;ZHOU Ting;ZENG Jingbin(State Key Laboratory of Heavy Oil Processing,College of Chemistry and Chemical Engineering,China University of Petroleum(East China),Qingdao 266580,Shandong,China;Qingdao Academy of Agricultural Sciences,Qingdao 266100,Shandong,China)

机构地区：[1]中国石油大学(华东)化学化工学院,重质油国家重点实验室,山东青岛266580 [2]青岛市农业科学研究院,山东青岛266100

出　　处：《实验室研究与探索》2022年第6期6-10,15,共6页Research and Exploration In Laboratory

基　　金：国家自然科学基金面上项目(21876206);山东省自然科学基金面上项目(ZR2020MB064);山东省重大基础研究项目(ZR2020ZD13);青岛市科技惠民示范引导专项(21-1-4-sf-7-nsh);中国石油大学教学改革项目(SJ-202036)。

摘　　要：利用柠檬酸钠还原法制得纳米金,在其上分别修饰与乙肝病毒核酸互补的两条DNA片段,构建生物探针,然后利用DNA片段与目标病毒核酸通过碱基互补配对进行杂交反应,形成纳米金团聚,产生颜色变化,实现乙肝病毒核酸的比色检测。该方法操作简便快速,只需5 min即可完成检测,具有良好的特异性和重现性,检测限可达0.66 nmol/L。The gold nanoparticles are prepared with sodium citrate reduction method.Further,by respectively modified with the two complementary DNA sequences of hepatitis B virus nucleic acid,the bionanoprobes are constructed to hybridize with target nucleic acid according to the complementary matching principle.The hybridization reaction induces aggregation of the gold nanoparticles,and it results in color change.Based on the result,the detection of hepatitis B virus nucleic acid is achieved.This method is convenient and rapid,and can be completed in only 5 minutes.Besides,it has good specificity and reproducibility,and the detection limit is up to 0.66 nmol/L.

关 键 词：纳米金 病毒 核酸 检测 综合性实验 

分 类 号：O652.6[理学—分析化学] G642[理学—化学]