Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock  

作　　者：ZHANG Ming-qing ZHANG Qiang YUAN Wei WU Jun-yuan LIANG Yong QIN Hong-jie LI Chun-sheng 

机构地区：[1]Department of Emergency Medicine,Beijing Friendship Hospital,Capital Medical University,Beijing,100050,China [2]Department of Critical Care Medicine,Peking University Third Hospital,Beijing,100191,China [3]Department of Emergency Medicine,Beijing Chaoyang Hospital,Capital Medical University,Beijing,100020,China [4]Department of Emergency Medicine,Beijing Luhe Hospital,Capital Medical University,Beijing,101199,China

出　　处：《Chinese Journal of Integrative Medicine》2022年第9期794-801,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81372025);the 2015 Annual Special Cultivation and Development Project for the Technology Innovation of Beijing(No.Z151100001615056)。

摘　　要：Objective:To investigate the effects of Shenfu Injection(SFI)on endothelial damage in a porcine model of hemorrhagic shock(HS).Methods:After being bled to a mean arterial pressure of40±3 mm Hg and held for 60 min,32 pigs were treated with a venous injection of either shed blood(transfusion group),shed blood and saline(saline group),shed blood and SFI(SEI group)or without resuscitation(sham group).Venous blood samples were collected and analyzed at baseline and 0,1,2,4,and 6 h after HS.Tumor necrosis factor-α(TNF-α),serum interleuking(IL)-6,and IL-10 levels were measured by enzymelinked immunosorbent assay(ELISA);expressions of vascular cell adhesion molecule-1(VCAM-1),intercellular adhesion molecule 1(ICAM-1),von Willebrand factor(vWF),plasminogen activator inhibitor-1(PAI-1)and Bcl-2,Bax,and caspase-3 proteins were determined by Western blot.Results:The serum level of TNF-αin the SFI group was significantly lower than in the other groups at 0,1,and 2 h after HS,while the level of IL-6 was lower at 4 and 6 h compared with the saline group(P<0.01 or P<0.05).The concentration of serum IL-10 was significantly higher in the SFI group than in the other groups at 0,1,4,and 6 h after HS(P<0.01).Western blot and immunohistochemistry of vascular tissue showed that the expression of caspase-3 was downregulated,and that of Bcl-2 and Bax was upregulated in the SFI group compared to other groups(P<0.05).Conclusion:SFI attenuated endothelial injury in the porcine model of HS by inhibiting cell apoptosis,suppressing the formation of proinflammatory cytokines,and reducing endothelial activation.

关 键 词：hemorrhagic shock Shenfu Injection vascular endothelial damage Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]