间歇性缺氧大鼠术后认知功能损伤模型的建立  

Establishment and assessment of postoperative cognitive impairment in intermittent hypoxia rats

作　　者：梅喜[1] 汪赛赢[1] MEI Xi;WANG Saiying(Department of Anesthesiology,Third Xiangya Hospital,Central South University,Changsha 410013,China)

机构地区：[1]中南大学湘雅三医院麻醉科,长沙410013

出　　处：《中南大学学报（医学版）》2022年第8期1136-1142,共7页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(2021JJ40940)。

摘　　要：目的:阻塞型睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)是一种以呼吸暂停或低呼吸反复发作为特征,伴有间歇性觉醒和睡眠干扰的慢性疾病,其发病率逐年增加,已成为严重危害人类生命健康的疾病。OSAHS与术后神经认知障碍密切相关,制备间歇性缺氧动物术后认知功能损伤模型是研究其发病机制和干预靶点的重要途径。本研究探讨间歇性缺氧大鼠术后认知功能损伤动物模型的建立及评价方法。方法:选取108只雄性SD大鼠,随机分为8组:对照组(C组,n=27)、手术组(S组,n=27)、间歇性缺氧7 d组(H1组,n=9)、间歇性缺氧14 d组(H2组,n=9)、间歇性缺氧21 d组(H3组,n=9)、间歇性缺氧7 d手术组(O1组,n=9)、间歇性缺氧14 d手术组(O2组,n=9)和间歇性缺氧21 d手术组(O3组,n=9)。H1、H2和H3组大鼠分别接受间歇性缺氧7、14和21 d处理,O1、O2和O3组大鼠分别在间歇性缺氧7、14和21 d后接受左肝外叶切除术。各组大鼠进行行为学测试,行为学测试的顺序为:旷场实验、新物体识别实验及巴恩斯迷宫实验。检测各组大鼠海马IL-1βm RNA的表达水平。结果:与C、S和H2组相比,O2组大鼠在术后6 h和术后第1天的辨别指数明显减低(P<0.05),术后第1和2天在巴恩斯迷宫实验中的犯错次数和找到安全盒时间明显增加(P<0.0001),术后第1天海马区IL-1βmRNA的表达水平明显增加(P<0.05)。而O1和O3组分别与H1和H3组比较,术后6 h和术后第1天辨别指数、巴恩斯迷宫实验中的犯错次数和找到安全盒时间以及术后第1天海马区IL-1βmRNA的表达水平差异均无统计学意义(均P>0.05)。结论:间歇性缺氧14 d大鼠再接受麻醉和手术后能建立术后认知功能损伤的动物模型。Objective:Obstructive sleep apnea hypopnea syndrome(OSAHS)is a chronic disease characterized by repeated episodes of apnea or hypopnea,accompanied by intermittent awakening and sleep disturbances.The incidence of OSAHS is increasing and has become a serious disease.In recent years,more and more evidence shows that OSAHS is closely related to postoperative neurocognitive disorders,and the preparation of models of postoperative cognitive impairment in intermittent hypoxia animals is an important way to study its pathogenesis and intervention targets.This study aims to explore the establishment and evaluation of the animal model of postoperative cognitive impairment in intermittent hypoxia rats.Methods:A total of 108 male SD rats were randomly divided into 8 groups:a control group(C group,n=27),a surgery group(S group,n=27),an intermittent hypoxia 7 d group(H1 group,n=9),an intermittent hypoxia 14 d group(H2 group,n=9),an intermittent hypoxia 21 d group(H3 group,n=9),an intermittent hypoxia 7 d operation group(O1group,n=9),an intermittent hypoxia 14 d operation group(O2 group,n=9),and an intermittent hypoxia 21 d operation group(O3 group,n=9).The rats in the H1,H2 and H3group treated with intermittent hypoxia for 7,14,and 21 d,respectively.The rats in the O1,O2 and O3 groups received left lateral hepatic lobectomy after 7,14,and 21 d intermittent hypoxia,respectively.The rats in each group were subjected to open field test,new object recognition test,and Barnes Maze test.The expression of IL-1βmRNA in hippocampus of rats was detected at the 1st day after the surgery.Results:Compared with the C,S,and H2 groups,the discrimination index in novel object recognition test 6 h and 1 d after the surgery of the O2 group was significantly lower(P<0.05),the latency and errors in Barnes maze at the 1st day and 2nd day after the surgery were increased significantly(P<0.05)and the expression of IL-1βmRNA in hippocampus was significantly increased at the 1st day after the operation(P<0.05).However,there was no difference in the

关 键 词：阻塞型睡眠呼吸暂停低通气综合征 间歇性缺氧 术后认知功能损伤 动物模型 

分 类 号：R766[医药卫生—耳鼻咽喉科]