白边侧足海天牛全基因组Survey分析  

Whole genome survey analysis of Elysia leucolegnote

作　　者：李歆毓 丁梦莹 冯尔辉 张源源[4,5] 章可兰 万迎朗 LI Xinyu;DING Mengying;FENG Erhui;ZHANG Yuanyuan;ZHANG Kelan;WAN Yinglang(Hainan Dongzhaigang National Natural Reserve Authority,Haikou,Hainan570100;College of Marine Biology,Hainan University,Haikou,Hainan 570228;College of Tropical Crops,Hainan University,Haikou,Hainan 570228;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101;Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China)

机构地区：[1]海南东寨港国家级自然保护区管理局,海口570100 [2]海南大学海洋学院,海口570228 [3]海南大学热带作物学院,海口570228 [4]中国热带农业科学院橡胶研究所,海口571101 [5]农业农村部橡胶树生物学与遗传资源利用重点实验室,海口571101

出　　处：《热带生物学报》2022年第5期457-463,共7页Journal of Tropical Biology

基　　金：海南省自然科学自然科学基金(No.2019RCI55)。

摘　　要：为揭示白边侧足海天牛基因组的信息,以便为后续构建全基因组精细图谱提供基础,利用流式细胞术与高通量测序技术对白边侧足海天牛全基因组进行了Survey分析。结果表明,流式细胞术预测白边侧足海天牛基因组是84K杨的1.69倍,约为794.6 Mb。白边侧足海天牛高通量测序得到25 Gb的过滤数据,总测序深度约为30 x,根据17-mer分析,预测海天牛基因组大小约为724.8 Mb,基因组重复率为52.8%,杂合度为1.55%,模型拟合值为99.38%;根据19-mer分析,预测海天牛基因组大小约为730.8 Mb,基因组重复率为35.1%,杂合度为1.68%,模型拟合值为99.72%。最后,对白边侧足海天牛尝试初步组装后得到总长为628Mb的基因组。研究结果显示,白边侧足海天牛是一个高度杂合的物种,且其基因组大小超过700 Mb。Elysia leucolegnote are sacoglossan sea slugs,belonging to the family Elysiidae and the genus Elysia.They are distributed in mangroves throughout China,Thailand and the Philippines.Photosynthetic sea slugs are famous for their ability of photosynthesis.Stolen chloroplasts are called kleptoplasts.However,there has been no important breakthrough in the molecular mechanism research of kleptoplasts.In this context whole genome survey analysis of E.leucolegnote was conducted to find the clue about the regulation of photosynthesis and maintenance of chloroplast activities by high-throughput sequencing technology.The highthroughput sequencing produced 25 Gb high-quality data in E.leucolegnote,with a total sequencing depth of about 30x.According to 17-mer analysis,the predicted genome size was 724.8 Mb,the genome duplication rate was 52.8%,the heterozygosity was 1.55%,and the model fitting value was 99.38%.The 19-mer analysis predicted that the genome size was 730.8 Mb,with the genome duplication rate of 35.1%,the heterozygosity of1.68%,and the model fitting value of 99.72%.A draft genome of E.leucolegnote Ker was obtained by preliminary assembly with a total length of 628 Mb.These results showed that E.leucolegnote is a highly heterozygous species with a genome size of more than 700 Mb.

关 键 词：白边侧足海天牛 基因组Survey分析 基因组大小 杂合度 

分 类 号：Q789[生物学—分子生物学] Q959.1