豆腐果苷对骨关节炎大鼠软骨和滑膜组织影响  

Effect of helicid on cartilage and synovium tissue of osteoarthritis rats

作　　者：郭南 贾元威[2] 项婷 王萍 詹翠娇 王昌茂 沈杰 杨斌[2] GUO Nan;JIA Yuanwei;XIANG Ting;WANG Ping;ZHAN Cuijiao;WANG Changmao;SHEN Jie;YANG Bin(School of Pharmacy,Wannan Medical College,Wuhu,Anhui 241002,China;Department of Clinical Pharmacy,The First Affiliated Hospital of Wannan Medical College,Wuhu,Anhui 241001,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002 [2]皖南医学院弋矶山医院临床药学部,安徽芜湖241001

出　　处：《安徽医药》2022年第10期1915-1919,I0001,共6页Anhui Medical and Pharmaceutical Journal

基　　金：皖南医学院弋矶山医院引进人才专项基金资助项目(YR201913,YR202015);皖南医学院弋矶山医院科研能力“高峰”培育计划精英基金资助项目(GF2019J05,GF2019J06);皖南医学院弋矶山医院科技创新团队“攀峰”培育计划’项目(KPF2019016);皖医中青年基金(WK2018F04)。

摘　　要：目的研究豆腐果苷对骨关节炎大鼠软骨结构和滑膜组织的影响。方法碘乙酸钠注射SD雄性大鼠膝关节造模成功后,随机区组分组法分为三组,即假手术组、模型组、豆腐果苷50 mg·kg^(−1)·d^(−1)组。豆腐果苷灌胃21 d后,取大鼠膝关节制成组织切片,HE染色观察膝关节软骨和滑膜病理变化,并进行评分;番红固绿染色观察大鼠膝关节软骨病理变化,并进行Mankin评分。研究起止时间为2019年12月至2020年7月。结果相对于假手术组,模型组大鼠膝关节软骨和滑膜均受到不同程度损伤,滑膜病理评分显著升高[(6.88±0.83)分比(0.50±0.53)分](P<0.01),且软骨结构、软骨细胞和番红固绿染色的Mankin评分也显著升高[分别是(3.88±0.83)分比(0.50±0.53)分,(1.75±0.46)分比(0.25±0.46)分,(2.38±0.74)分比(0.38±0.52)分](P<0.01)。然而,连续给予豆腐果苷灌胃21 d后,相对于模型组,大鼠的关节软骨结构和滑膜组织损伤程度均有显著改善,膝关滑膜病理评分显著降低[(3.50±0.53)分比(6.88±0.83)分](P<0.01),且软骨结构、软骨细胞和番红固绿染色的Mankin评分也显著降低[分别为(1.63±0.52)分,(1.13±0.64)分,(0.88±0.64)分](P<0.05)。结论豆腐果苷可减轻骨关节炎大鼠膝关节软骨和滑膜损伤,具有减轻滑膜炎症、保护软骨的作用。Objective To evaluate the effects of helicid on pathologic changes of cartilage and synovium in osteoarthritis(OA)rats.Methods After the knee joints of SD male rats were successfully molded by sodium iodoacetate injection,they were random block grouping assigned into three groups,namely,sham-operated group,model group,and helicid 50 mg·kg^(−1)·d^(−1) group.After 21 days of helicid gavage,tissue sections were taken from the knee joints of rats,and the pathological changes of knee cartilage and synovial membrane were observed by hematoxylin&eosin(HE)staining and scored;the pathological changes of knee cartilage of rats were observed by Safranin O-Fast Green staining and scored by Mankin.The study starts and ends from December 2019 to July 2020.Results Compared with the sham-operated group,the knee cartilage and synovium of the model group were damaged to different degrees,and the synovial pathologcal scores were significantly higher[(6.88±0.83)vs.(0.50±0.53)](P<0.01),and Mankin scores for cartilage structure,chondrocytes,and Safranin O-Fast Green staining were significantly higher[(3.88±0.83)vs.(0.50±0.53),(1.75±0.46)vs.(0.25±0.46),and(2.38±0.74)vs.(0.38±0.52),respectively](P<0.01).However,after 21 days of continuous administration of helicid gavage,compared with the model group,the degree of damage to articular cartilage structures and synovial tissues in the rats was significantly improved,with a significant decrease in the knee joint synovial pathological score[(3.50±0.53)vs.(6.88±0.83)](P<0.01)and a significant decrease in the Mankin scores for cartilage structure,chondrocytes and Safranin O-Fast Green staining[(1.63±0.52),(1.13±0.64),and(0.88±0.64),respectively](P<0.05).Conclusion Helicid could reduce the injury of cartilage structure and the synovial tissue in knee joints of rats with osteoarthritis and has the effect of reducing synovial inflammation and protecting cartilage.

关 键 词：山龙眼科 骨关节炎 膝 豆腐果苷 软骨 滑膜 大鼠 Sprague-Dawley 

分 类 号：R285.5[医药卫生—中药学]