急性支气管哮喘病人外周血程序性死亡因子-1及其配体的表达  

Expression of programmed death factor-1 and its ligands in peripheral blood of patients with acute bronchial asthma

作　　者：钱美芳 张艳 尤倩 赵薇 QIAN Meifang;ZHANG Yan;YOU Qian;ZHAO Wei(Department of Respiratory and Critical Care Medicine,Wuxi Fifth People's Hospital,Wuxi,Jiangsu 214000,China)

机构地区：[1]无锡市第五人民医院呼吸与危重症医学科,江苏无锡214000

出　　处：《安徽医药》2022年第10期2005-2009,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨急性支气管哮喘病人外周血程序性死亡因子-1(PD-1)及其配体PD-L1的表达水平及意义。方法选择2017年2月至2019年3月无锡市第五人民医院收治的101例急性支气管哮喘病人作为病例组,同期体检正常40例健康者为对照组;病例组病人分为轻中度组46例、重度组34例、危重组21例。采用流式细胞术法检测外周血CD4+T淋巴细胞中PD-1表达(CD4+PD-1)、CD8+T淋巴细胞中PD-1表达(CD8+PD-1),CD19+B淋巴细胞中PD-L1(CD19+PD-L1)表达,采用单因素方差分析其与疾病严重程度的关系;采用化学发光分析仪检测呼出气一氧化氮(FeNO)水平,酶联免疫吸附法(ELISA)检测外周血白细胞介素4(IL-4)、白细胞介素17(IL-17)水平。结果病例组外周血CD4+PD-1、CD19+PD-L1表达水平及PD-1/PD-L1值均高于对照组高[17.98±4.16比5.43±1.52、3.13±0.75比1.08±0.27、5.75±1.62比5.03±1.11](P<0.05);病例组FeNO及外周血IL-17水平高于对照组[(29.74±6.08)μg/L比(16.38±4.12)μg/L、(38.66±8.27)ng/L比(24.73±5.21)ng/L],外周血IL-4水平低于对照组[(9.14±2.47)ng/L比(12.86±3.65)ng/L](P<0.05);病例组不同严重程度外周血CD4+PD-1、CD19+PD-L1表达水平及PD-1/PD-L1值由高至低为危重组、重度组、轻中度组(P<0.05);病例组外周血CD4+PD-1、CD19+PD-L1与FeNO呈正相关关系(r=0.61、0.55,P<0.05),与IL-17呈正相关关系(r=0.51、0.50,P<0.05),与IL-4呈负相关关系(r=−0.43、−0.38,P<0.05)。结论急性支气管哮喘病人外周血PD-1/PD-L1(CD4+PD-1/CD19+PD-L1)表达异常,病情越严重,其表达水平可能越高。Objective To investigate the expression and significance of programmed death factor-1(PD-1)and its ligand PD-L1 in peripheral blood of patients with acute bronchial asthma.Methods Totally 101 cases of acute bronchial asthma admitted to Wuxi Fifth People's Hospital from February 2017 to March 2019 were selected as case group,and 40 healthy subjects were enrolled in the control group.Patients in the case group were subdivided into mild to moderate group(n=46),severe group(n=34)and critical group(n=21).The expressions of PD-1 in peripheral blood CD4+T lymphocytes(CD4+PD-1),CD8+T lymphocytes(CD8+PD-1),and CD19+B lymphocytes(CD19+PD-L1)were detected by flow cytometry and the relationship between PD-1 expression and disease severity was analyzed by one-way ANOVA.The level of exhaled nitric oxide(FeNO)was detected by chemiluminescence analyzer,and the levels of interleukin-4(IL-4)and interleukin-17(IL-17)in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).Re⁃sults The expression levels of CD4+PD-1,CD19+PD-L1 and PD-1/PD-L1 in peripheral blood of the case group was higher than those of the control group[(17.98±4.16)vs.(5.43±1.52),(3.13±0.75)vs.(1.08±0.27),(5.75±1.62)vs.(5.03±1.11);P<0.05].The FeNO level and peripheral blood IL-17 level in the case group were higher than those in the control group[(29.74±6.08)μg/L vs.(16.38±4.12)μg/L,(38.66±8.27)ng/L vs.(24.73±5.21)ng/L;P<0.05],while the level of IL-4 in the case group was lower than that in the control group[(9.14±2.47)ng/L vs.(12.86±3.65)ng/L,P<0.05].The expression levels of CD4+PD-1,CD19+PD-L1 and PD-1/PD-L1 in peripheral blood of patients with different severity from high to low were found in critical group,severe group and mild-to-moderate group(P<0.05).In the case group,CD4+PD-1 and CD19+PD-L1 were positively correlated with FeNO(r=0.61,0.55,respectively;P<0.05),positively correlated with IL-17(r=0.51,0.50,respectively;P<0.05),and negatively correlated with IL-4(r=−0.43,−0.38,respectively;P<0.05).Conclusion The expressio

关 键 词：哮喘 程序性死亡因子-1 程序性死亡配体-1 呼出气一氧化氮 CD4淋巴细胞计数 抗原 CD19 

分 类 号：R562.25[医药卫生—呼吸系统]