栽培种花生LEA蛋白基因家族的鉴定及其低温胁迫下表达分析  

Genome-wide Identification and Expression Analysis of the Late Embryogenesis Abundant(LEA)Protein Gene Family in Cultivated Peanut(Arachis hypogaea L.)on Its Response to Cold Stress

作　　者：付娆 李佳佳 单燕 张海洋 衣葵花[1] 修妤[1] 梁晓艳[1] 张佳蕾[2] FU Rao;LI Jia-jia;SHAN Yan;ZHANG Hai-yang;YI Kui-hua;XIU Yu;LIANG Xiao-yan;ZHANG Jia-lei(Shandong Institute of Sericulture,Yantai 264002,China;Institute of Crop Germ plasm Resources,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东省蚕业研究所,山东烟台264002 [2]山东省农业科学院农作物种质资源研究所,山东济南250100

出　　处：《花生学报》2022年第3期1-12,共12页Journal of Peanut Science

基　　金：国家重点研发课题(2020YFD1000905);山东省农业科学院创新工程项目(CXGC2022A10,CXGC2022E19;CXGC2022F06);山东省自然科学青年基金项目(ZR2020QC062);山东省“渤海粮仓”科技示范工程项目(2019BHLC004)。

摘　　要：胚胎发育晚期富集蛋白(LEA)在植物胚胎发育过程和抵御非生物胁迫中起重要作用。为了探索栽培种花生LEA蛋白家族基因(AhLEA)的结构和功能,利用生物信息学方法对AhLEA基因家族成员进行全基因组鉴定,并对其种子发育不同时期和低温胁迫下的表达特性进行分析。结果表明:在栽培种花生中共鉴定到118个AhLEA基因,根据其保守结构域和进化关系分为八个亚家族,LEA_2亚家族成员最多;大多数AhLEA蛋白是亲水的,定位于细胞核、叶绿体和细胞膜;87%AhLEA基因具有较少的内含子(<3),并且在所有20条染色体上分布不均。转录组数据显示,在低温胁迫(2℃)条件下,45个AhLEA基因表达发生变化,其中,28个基因表达量升高,17个基因表达量降低。对表达显著上调的9个AhLEA基因进行qRT-PCR验证,其表达趋势与转录组结果相一致。在种子发育过程中表达量升高的16个基因及表达量降低的2个基因,在低温胁迫条件下表达量均显著增加,预示着这18个AhLEA基因在调控花生种子发育及对低温胁迫的响应中可能发挥着重要作用,为进一步研究AhLEA基因在花生种子萌发期抵抗低温胁迫中的作用提供了基础数据。Late embryogenesis abundant(LEA)proteins are considered to play important functional roles in embryonic development and abiotic stress responses.In order to explore the structure and function of AhLEA gene family in cultivated peanut,the comprehensive genome-wide identification and bioinformatics analysis were conducted,and the gene expression were analyzed during seed development and under cold stress.In this study,a total of 118 AhLEA genes were identified from cultivated peanut,and the identified genes were classified into eight groups based on their conserved domain and evolutionary relationship.Genes in the LEA_2 group were found to be the most abundant.Most of the AhLEA proteins were hydrophilic and localized in the nucleus,chloroplasts and cytomembrane of the cell.87%of AhLEA genes had a few of introns(<3)and were unevenly distributed in all 20 chromosomes.RNA-seq data demonstrated that the expression of 45 AhLEA genes were varied under cold stress(2℃).Among them,the expression of 28 AhLEA genes increased,while 17 AhLEA genes decreased.Subsequently,qRT-PCR of nine AhLEA genes with significantly up-regulated expression was carried out,and the expression pattern was consistent with the results of RNA-Seq data.The expression of 16 AhLEA genes with increased expression and 2 AhLEA genes with decreased expression during seed development all increased significantly under cold stress,indicating that these 18 AhLEA genes may play important roles in regulating seed development and responding to cold stress in peanut.These results provide fundamental insights for further study on the resistance role of AhLEA gene to low temperature stress during peanut seed germination period.

关 键 词：栽培种花生 LEA基因家族 全基因组鉴定 低温胁迫 表达分析 

分 类 号：Q786[生物学—分子生物学] S565.2[农业科学—作物学]