花生乙醇酸氧化酶基因鉴定及其响应非生物胁迫的表达分析  

Identification of Glycolate Oxidase Gene and Expression Analysis on Its Responding to Abiotic Stresses in Peanut

作　　者：梁天柱 程凤玲 张晓军 邹晓霞 司彤 王月福 于晓娜 LIANG Tian-zhu;CHENG Feng-ling;ZHANG Xiao-jun;ZOU Xiao-xia;SI Tong;WANG Yue-fu;YU Xiao-na(College of Agronomy,Qingdao Agricultural University/Shandong Key Lab.of Dry Farming Techniques/Shandong Provincial Peanut Industry Coo perative Innovation Center,Qingdao 266109,China)

机构地区：[1]青岛农业大学农学院/山东省旱作农业技术重点实验室/山东省花生产业协同创新中心,山东青岛266109

出　　处：《花生学报》2022年第3期13-20,27,共9页Journal of Peanut Science

基　　金：山东省农业良种工程(2020LZGC001);山东省花生现代产业技术体系(SDAIT-04-05);国家花生产业技术体系建设项目(CARS-13-生态与土壤管理)。

摘　　要：花生是我国重要的油料和经济作物,非生物胁迫严重影响其产量和品质。乙醇酸氧化酶(Glycolate Oxidase,GOX)不仅是光呼吸途径中的关键酶,同时在植物响应非生物胁迫中发挥重要作用。本研究利用生物信息学的方法,在栽培花生A.hypogaea(2n=40,AABB)全基因组水平上鉴定到了16个GOXs基因家族成员,在野生二倍体花生A.duranensis(2n=20,AA)和A.ipaensis(2n=20,BB)全基因组水平上分别鉴定到了9个和5个GOXs基因家族成员。基因结构分析发现,该基因家族成员由4~20个外显子构成。通过进化树分析,可将花生GOXs基因家族成员分为5个组。以耐盐品种花育25号和盐敏感品种花育20号为材料,进行干旱、盐、高温和低温非生物胁迫处理,根据qRT-PCR分析结果表明,在两个品种和不同组织之间的表达量具有显著差异。本研究为进一步研究花生响应非生物胁迫的机制提供了候选基因。Peanut is one of the economically important oil crops in China.Abiotic stresses affect the yield and quality of peanut crop.Glycolate Oxidases(GOXs)are one of the most important classes of enzymes that are involved in the photorespiration pathway and plays key roles during abiotic stress responses in plant.In this study,16 genes of the GOXs family were identified in cultivated peanut A.hypogaea(2n=40,AABB)genome based on bioinformatics method.Besides,9 and 5 genes were also identified from two wild diploid peanut genomes,A.duranensis(2n=20,AA)and A.ipaensis(2n=20,BB)respectively.Gene structure analysis showed that the GOXs genes are composed of 4 to 20 exons.The GOXs genes were grouped into 5 clusters by phylogenetic tree analysis.Additionally,Huayu20(HY20),salt-susceptible variety,and Huayu25(HY25),salt-tolerant variety,were selected as research materials.The quantitative real-time PCR revealed that GOXs genes from different groups exhibited expression variation in different tissues under drought,salt,heat,and cold stress treatments.These results can provide candidate genes for further functional characterization of GOXs genes responding to abiotic stress in peanut crop.

关 键 词：花生 乙醇酸氧化酶 非生物胁迫 基因表达 

分 类 号：Q786[生物学—分子生物学] S565.2[农业科学—作物学]