Development of a chromatographic lateral flow immunoassay for detection of African swine fever virus antigen in blood  

作　　者：Daniel W.Madden Sun-Young Sunwoo Natasha N.Gaudreault Jessie D.Trujillo Igor Morozov Carmina Gallardo Juergen A.Richt 

机构地区：[1]Department of Diagnostic Medicine/Pathobiology,College of Veterinary Medicine,Kansas State University,Manhattan,KS,USA [2]Centro de Investigación en Sanidad Animal(CISA-INIA/CSIC),Madrid,Spain

出　　处：《Animal Diseases》2022年第3期139-153,共15页动物疾病（英文）

基　　金：Funding for this study was provided through grants from the National Bio and Agro-Defense Facility(NBAF)Transition Fund from the State of Kansas,and the AMP Core of the Center of Emerging and Zoonotic Infectious Diseases(CEZID)from National Institute of General Medical Sciences(NIGMS)under award number P20GM130448.

摘　　要：African swine fever(ASF)is a highly lethal disease of domestic and wild swine caused by African swine fever virus(ASFV).The disease currently circulates in Africa,Europe,Asia and on the island of Hispaniola.The ongoing epizootics in Europe and Asia have produced millions of animal deaths and severe economic losses.No effective vaccine is available for ASF,making rapid and accurate detection of ASFV essential for disease mitigation strategies.Currently available diagnostics for ASFV possess significant limitations related to assay performance,deployability,and/or turn-around time;therefore there is an unmet need for pen-side diagnostic tests with sufficient sensitivity and specificity.A chromatographic lateral flow immunoassay(LFIA)was developed for the detection of ASFV antigen in EDTA-treated whole blood using monoclonal antibodies targeting the viral p30 protein.The assay requires only water to perform and provides results in 25 min,making it well-suited for field use.The LFIA was capable of detecting genotype I and genotype II strains of ASFV in EDTA blood from experimentally infected pigs at varying time-points after infection,though it was unable to detect a genotype X ASFV strain.Diagnostic sensitivity correlated with clinical disease severity,body temperature,and viral DNA levels,and was over 90%in animals showing moderate to severe ASF-related symptoms after challenge with virulent genotype II virus.The LFIA also showed a robust diagnostic specificity of over 98%,which is essential to field testing for a high consequence to foregin animal disease.The LFIA targeting the viral p30 protein can reliably detect ASFV in whole blood from animals showing moderate to severe clinical signs of infection with virulent genotype I and II isolates,making it a promising candidate for use as a field-deployable antigen detection assay.Additional evaluation using field samples and different virus strains is required to further assess the utility of this rapid diagnostic test.

关 键 词：African swine fever Lateral flow immunoassay Point of care diagnostics 

分 类 号：S852.65[农业科学—基础兽医学]