薯莨单宁的提取及其抑菌活性研究  

Tannic Acid Extraction From Dioscorea cirrhosa and Antimicrobial Activity of D. cirrhosa Extract

作　　者：李落叶 杨晓朱 雷梦英 梁传冠 何旭君 黄少彬 LI Luoye;YANG Xiaozhu;LEI Mengying;LIANG Chuanguan;HE Xujun;HUANG Shaobin(Guangdong Eco-Engineering Polytechnic/Collaborative Innovation Center of Plant Pest Management and Bioenvironmental Health Application Technology,Guangzhou,Guangdong 510520,China;Huizhou Taihe Xinchuang Technology Co.,Ltd.,Huizhou,Guangdong 516000,China)

机构地区：[1]广东生态工程职业学院/植物有害生物防控与生物环境健康应用技术协同创新中心,广东广州510520 [2]惠州泰合新创科技有限公司,广东惠州516000

出　　处：《林业与环境科学》2022年第3期80-85,共6页Forestry and Environmental Science

基　　金：广东省教育厅特色创新项目(2018GKTSCX006);广东省林业科技创新项目(2019kjcx022)。

摘　　要：为了更好地利用薯莨Dioscorea cirrhosa资源,减少薯莨渣浪费,从薯莨和薯莨渣中提取生物活性成分,比较了薯莨提取物对几种重要植物病原菌的抑菌作用。结果显示,60%丙酮浸提法比75%乙醇提取法能获得较多提取物,且提取物中单宁含量较高,适合用于薯莨单宁提取。相同提取条件下,薯莨渣的浸提物得率比薯莨低50%,但两种提取物中单宁含量无明显差异。抑菌试验结果表明,薯莨的丙酮提取物对来自猴耳环Pithecellobium clypearia的炭疽病菌(暂定名为H30,Colletotrichum sp.)、拟茎点霉(暂定名为H63,Phomopsis sp.)和广藿香青枯病菌(暂定名为GHB2,Ralstonia sp.)均有明显的抑制效果。因此,薯莨和薯莨渣可望成为研制新型植物源杀菌剂的原料,为药用植物病害绿色防控提供新制剂。For fully utilization of Dioscorea cirrhosa and its residues from the manufacturing process of Gambiered Guangdong silk, biologically active substance was tried to be extracted and the antimicrobial activity of D. cirrhosa extracts was tested. The results showed that more extracts were obtained and more tannic was in the extracts by 60% acetone than by 75% ethanol. It is suitable to extract tannic from D. cirrhosa using 60%acetone. Using the same extraction method, the output rate of extracts from D. cirrhosa residues was 50% lower than from D. cirrhosa, but the tannic acid content in extracts from both of them were very similar. The extracts of D. cirrhosa. from 60% acetone showed high antimicrobial activity to a species of Colletotrichum and a species of Phomopsis from Pithecellobium clypearia. It also had obvious antibacterial activity to Ralstonia solanacearum of Pogostemon cablin. So, It is possible to develop a new botanical germicide using D. cirrhosa and its residues and provide a new preparation for nuisanceless control of medicinal plant diseases.

关 键 词：薯莨 单宁 杀菌剂 

分 类 号：S763.11[农业科学—森林保护学]