干旱胁迫对屋顶绿化植物小叶黄杨叶片抗氧化特性的影响  

Effects of Drought Stress on Antioxidant Properties of Roof Greening Plant Buxus microphylla Leaves

作　　者：刘慧 张崇洋 刘世亮[1] 周孟椋 吴苗苗 姜桂英[1] 刘芳[1] 赵颖[1] LIU Hui;ZHANG Chongyang;LIU Shiliang;ZHOU Mengliang;WU Miaomiao;JIANG Guiying;LIU Fang;ZHAO Ying(College of Resources and Environment,Henan Agricultural University,Zhengzhou,Henan 450002,China)

机构地区：[1]河南农业大学资源与环境学院,河南郑州450002

出　　处：《林业与环境科学》2022年第3期86-93,共8页Forestry and Environmental Science

摘　　要：以2年生小叶黄杨Buxus microphylla叶片为试材,通过盆栽试验模拟自然干旱条件,设置正常供水(田间持水量的80%~90%)、轻度干旱胁迫(田间持水量的60%~70%)、中度干旱胁迫(田间持水量的50%~60%)和重度干旱胁迫(田间持水量的35%~45%)4个处理,监测小叶黄杨不同生长时期的叶片相关酶活性,以期研究干旱胁迫条件下小叶黄杨叶片抗氧化特性的变化特征。研究发现:随着干旱胁迫程度的增加和时间的延长,叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)活性均呈升高-降低-升高趋势;丙二醛(MDA)含量呈先下降后上升趋势;过氧化氢酶(CAT)活性和可溶性蛋白质含量均呈先上升后下降趋势。可见,轻度干旱胁迫(TB)会使小叶黄杨叶片抗氧化酶活性增强和可溶性蛋白质含量升高,但重度干旱胁迫(TD)会损害其正常生长。结果表明:在轻度和中度干旱胁迫后,通过提高保护酶活性和可溶性蛋白质含量来协调和维持氧化物含量的相对平衡以保证小叶黄杨正常生长发育,而重度胁迫后,小叶黄杨保护酶系统和细胞遭受不可逆的损害,影响其正常生长。The pot experiment was carried out with taking the leaves of 2-year-old B. microphylla as test materials under simulated natural drought conditions. Four treatments of normal water supply(80%-90%field water capacity,CK), mild drought stress(60%-70% field water capacity,TB), moderate drought stress(50%-60% field water capacity,TC) and severe drought stress(35%-45% field water capacity,TD) were set to dynamically monitor the activities of related enzymes in leaves of B. microphylla at different growth stages, in order to study the changes characteristics of leaf antioxidant properties of B. microphylla which was a roof greening plant under drought stress.The study found that the activities of superoxide dismutase(SOD)and peroxidase(POD) in leaves were increased first, then decreased, and finally increased with drought stress degree and time.The content of malondialdehyde(MDA) in leaves of B. microphylla was decreased firstly and then increased. The activity of catalase(CAT) and the content of soluble protein were increased firstly and then decreased. So the activity of antioxidant enzymes and the content of soluble protein increased under mild drought stress, but the normal growth of B. microphylla would be damaged under severe drought stress.The results showed that the relative balance of oxide content was coordinated and maintained by increasing protective enzyme activity and soluble protein content to ensure the normal growth and development of B. microphylla after mild and moderate drought stress, while after severe stress, the protective enzyme system and cells of B. microphylla suffered irreversible damage and affected their normal growth.

关 键 词：小叶黄杨 屋顶绿化 干旱胁迫 叶片酶活性 抗氧化 

分 类 号：S181[农业科学—农业基础科学]