血管紧张素Ⅱ激活NLRP3炎性小体通路促进子宫内膜上皮细胞的转分化作用  

AngiotensinⅡactivates NLRP3 inflammasome pathway and promotes transdifferentiation of endometrial epithelial cells

作　　者：姜黎黎 单铁英[2] 李伟[3] 刘晓霞[2] 宋永红[2] 何红梅[2] JIANG Li-li;SHAN Tie-ying;LI Wei;LIU Xiao-xia;SONG Yong-hong;HE Hong-mei(Department of Gynecology.Handan Central Hospital,Handan Hebei,056002.China;Medicine College,Hebei Universiy of Engineering,Handan Hebei,056002,China;International Exchange College,Hebei Universiy of Engineering,Handan Hebei,056002,China)

机构地区：[1]邯郸市中心医院妇科,河北邯郸056002 [2]河北工程大学医学院,河北邯郸056002 [3]河北工程大学国际交流学院,河北邯郸056002

出　　处：《职业与健康》2022年第16期2194-2197,共4页Occupation and Health

摘　　要：目的分析NOD样受体蛋白3(NOD-like receptor protein 3,NLRP3)炎性小体在血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导的子宫内膜上皮细胞(endometrial epithelial cells,EECs)转化为间充质细胞中的作用及其机制。方法2021年2月1日—8月26日选取正常的子宫内膜,经酶消化并分离出EECs。在细胞培养液中加入浓度不等的AngⅡ作用不同时间,噻唑蓝染色分析细胞数量的改变;酶联免疫技术(enzyme linked immunosorbent assay,ELISA)检测细胞释放入培养液里的Ⅰ型、Ⅲ型胶原蛋白的含量;Western blot评估细胞表型蛋白平滑肌肌动蛋白-α(smooth muscle actin,α-SMA)和钙黏蛋白E(E-cadherin,E-cad)的水平、细胞内NLRP3、半胱氨酸蛋白酶-1(Caspase-1)和白介素-1β(interleukin-1β,IL-1β)的含量。结果与正常组相比,培养液中加入AngⅡ的量越多,作用时间越长,细胞的数量越多,以AngⅡ(10-6mol/L)培养48 h时细胞数量(0.121±0.002)增多最明显(P<0.01);AngⅡ组的细胞表型蛋白α-SMA含量(0.498±0.024)升高而E-cad含量(0.351±0.018)下降,释放Ⅰ型(0.805±0.074)、Ⅲ型(0.785±0.067)胶原蛋白的含量增多,细胞内NLRP3(0.556±0.020)、Caspase-1(1.123±0.049)和IL-1β(1.269±0.053)的含量增加(均P<0.05)。结论AngⅡ能促进EECs增殖、向间充质细胞转化以及分泌活力,与NLRP3炎症小体的激活关系密切。Objective To analyze the role and mechanism of NOD-like receptor protein 3(NLRP3)inflammatory bodies in the transformation of endometrial epithelial cells(EECs)into mesenchymal cells induced by angiotensinⅡ(AngⅡ).Methods The normal endometrium was selected from February 1 to August 26,2021,and EECs were isolated from it by enzyme digestion.AngⅡwith different concentrations was added to the cell culture medium for different times,and the changes of cell number were analyzed by thiazole blue staining.The enzyme linked immunosorbent assay(ELISA)was used to detect the content of typeⅠand typeⅢcollagen released into the culture medium.The levels of cell phenotypic proteins a-smooth muscle actin(α-SMA)and E-cadherin(E-cad),and the contents of intracellular NLRP3,Caspase-1 and interleukin-1β(IL-1β)were evaluated by Western blot.Results Contrast to the normal group,the more AngⅡwas added into the culture medium,the longer action time was,and the more cells were.When AngⅡ(10-6mol/L)was cultured for 48 h,the number of cells(0.121±0.002)increased most significantly(P<0.01).In AngⅡgroup,the content of phenotypic proteinα-SMA increased(0.498±0.024),while the content of E-cad decreased(0.351±0.018),the release of typeⅠ(0.805±0.074)and typeⅢ(0.785±0.067)collagen increased,and the contents of intracellular NLRP3(0.556±0.020),Caspase-1(1.123±0.049)and IL-1β(1.269±0.053)increased(all P<0.05).Conclusion AngⅡcan promote the proliferation,transformation to mesenchymal cells and secretory activity of EECs,which is closely related to the activation of NLRP3 inflammatory bodies.

关 键 词：血管紧张素Ⅱ 子宫内膜上皮细胞 NLRP3小体通路 转分化 

分 类 号：R446[医药卫生—诊断学]