宁夏老龄枸杞园撂荒过程中土壤理化性质和细菌群落变化特征  

Variation Characteristics of Physicochemical Properties and Bacterial Community During Restoration Progress of Soils in Abandoned Lycium Barbarum L.Orchards in Ningxia

作　　者：韩新宁[1] 张慧[2] 张俊华[3] 王芳[4] 李明[5] HAN Xin-ning;ZHANG Hui;ZHANG Jun-hua;WANG Fang;LI Ming(College of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan 756000,Ningxia,China;School of Mathematics and Computer Science,Ningxia Normal University,Guyuan 756000,Ningxia,China;College of Ecology and Environment,Ningxia University,Yinchuan 750021,China;College of Geographical Sciences and Planning,Ningxia University,Yinchuan 750021,China;School of Food and Wine,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏师范学院化学化工学院,宁夏固原756000 [2]宁夏师范学院数学与计算机科学学院,宁夏固原756000 [3]宁夏大学生态环境学院,银川750021 [4]宁夏大学地理科学与规划学院,银川750021 [5]宁夏大学食品与葡萄酒学院,银川750021

出　　处：《节水灌溉》2022年第9期39-45,共7页Water Saving Irrigation

基　　金：宁夏自然科学基金项目(2020AAC03268);国家自然科学基金项目(42067022);宁夏重点研发项目(2021BEF02016-1)。

摘　　要：选取宁夏老龄枸杞园撂荒前和撂荒后连续5年的土壤,利用高通量测序技术解析土壤(0-20和20-40cm)细菌群落结构,明确关键的土壤影响因子。结果表明:撂荒后20-40 cm土层含水量、有机质和碱解氮含量显著增加,含盐量、全氮、NH_(4)^(+)-N和NO_(3)^(-)-N含量显著降低。表土有机质、碱解氮和速效磷逐渐增加,全氮则逐渐减少,细菌多样性指数普遍逐渐增大。撂荒后Proteobacteria和Acidobacteriota相对丰度分别增加15.19%和28.38%,但Bacteroidota、Actinobacteriota和Cyanobacteria相对丰度分别减少26.72%、40.09%和46.13%。优势属Hymenobacter、Limnobacter和Cavicella数量增加了13.18、8.98和6.24倍,而Candidatus_Methylomirabilis和Flavisolibacter降低了91.37%和72.98%。Pearson相关分析表明撂荒过程中土壤全氮、含水率、速效磷、速效钾和NO_(3)^(-)-N是影响土壤细菌群落组成的关键因子。研究结果可为宁夏老龄枸杞撂荒园的管理与可持续利用提供科学依据。In this study,soils in abandoned Lycium barbarum L.orchard for five consecutive years before and after abandonment were collected,and high-throughtput sequencing technology was used to analyze the bacterial structure of soil(0-20 cm and 20-40 cm),as well as identified the critical soil factors.The results indicated that:compared to the control(before to abandonment)treatment,the soil water content organic matter,and alkali-hydrolyzable N content increased greatly in the fallow soil,while the soil salt content and total N content declined dramatically.Meanwhile,in the topsoil,soil organic matter,alkali-hydrolyzable nitrogen and available phosphorus increased gradually,tota nitrogen decreased gradually,and soil bacterial diversity indexes gradually increased.Compared with the control treatment,the abundance of Proteobacteria and Acidobacteriota increased 15.19%and 28.38%,respectively,while Bacteroidota,Actinobacteriota and Cyanobacteria decreased 26.72%,40.09%and 46.13%,respectively.At genus level,Hymenobacter,Limnobacter and Cavicella abundance increased by13.18,8.98 and 6.24-fold,while Candidatus_Methylomirabilis and Flavisolibacter decreased by 91.37%,and 72.98%,respectively.Pearson’s correlation analysis revealed that soil total N,water content,available P,available K and NO_(3)^(-)-N were the key environmental factors affecting soil bacterial community diversity and relative abundance of dominating phyla in the abandoned soils.This work can establish a scientific foundation for managing and reusing an abandoned Lycium barbarum L.orchard in Ningxia.

关 键 词：枸杞园 撂荒 土壤理化性质 细菌群落 相关性 

分 类 号：S154.36[农业科学—土壤学]