面向下一代数字病理成像分析仪的高通量全彩色傅里叶叠层显微成像术(特邀)  

High-throughput Full-color Fourier Ptychographic Microscopy for the Next Generation of Digital Pathologic Imager and Analyser(Invited)

作　　者：潘安[1,2] 高宇婷 王爱业 高慧琴 马彩文 姚保利[1,2] PAN An;GAO Yuting;WANG Aiye;GAO Huiqin;MA Caiwen;YAO Baoli(Xi'an Institute of Optics and Precision Mechanics,Chinese Academy of Sciences,Xi'an 710119,China;University of Chinese Academy of Sciences,Beijing 100094,China)

机构地区：[1]中国科学院西安光学精密机械研究所,西安710119 [2]中国科学院大学,北京100094

出　　处：《光子学报》2022年第7期191-215,共25页Acta Photonica Sinica

基　　金：National Natural Science Foundation of China(Nos. 12104500,12127805)。

摘　　要：傅里叶叠层显微成像术是近年来提出的新型计算成像技术,它有效解决了传统显微成像中分辨率与视场制约的问题,无需对样本进行机械扫描便能获得十亿像素级的高通量图像,有效解决传统数字病理扫描仪器的拼接伪影、重影、拼接成功率低、景深狭小、效率偏低等问题。近年来更是发现其不单是解决视场与分辨率制约的工具,更是解决一系列权衡问题的范式,从而迸发出源源不绝的生命力与应用潜力。本文全方位概述了傅里叶叠层显微成像术技术9个方面的发展趋势,简介了其起源与基本原理,着重综述了其在面向下一代数字病理成像分析仪的多个阶段与最新进展。指出其在这一应用方向上已进入“10-100”的产业化阶段。讨论了其产生大规模社会经济效益的可能性,其极有可能给数字病理行业及其上下游相关行业带来突破进展。尽管如此,作为典型交叉领域仍有不尽人意之处,包括科学问题、技术问题、工程问题及行业问题,需要多方共同努力推进,展望了未来技术与工程发展方向。Fourier Ptychographic Microscopy(FPM) is a promising computational imaging technique,which tackles the intrinsic trade-off between high resolution and large wide Field Of View(FOV)with a combination of Synthetic Aperture Radar(SAR) and optical phase retrieval. In brief,an LED array beneath the microscope provides illumination of the object from different incident angles. The range of light that can be collected is determined by the Numerical Aperture(NA)of the objective,while parts of the scattering light with a high-angle illumination can also be collected because of light-matter interaction. The low resolution intensity images recorded at each illumination angle are then synthesized in the Fourier domain,thus the object’s high-frequency information can be modulated into the passband of the objective.After an iterative phase reconstruction process,the synthesized information generates a high resolution object image including both intensity and phase properties. Additionally,it preserves the original large FOV as a low-NA objective is used to stitch low resolution images together. Given its flexible setup without mechanical scanning and interferometric measurement,FPM has developed rapidly,which not only acts as a tool to obtain both HR and large FOV but is also regarded as a paradigm to solve a series of trade-off problems,say,the trade-off between angular resolution and spatial resolution in light field imaging. And it may inspire to solve the trade-off between spectral resolution and spatial resolution in imaging spectrometer in the future.In this paper,we comprehensively summarized the development trend of FPM technique in 9 aspects,including high-precision imaging,high-throughput imaging,high-speed or single shot imaging,3D or tomography imaging, mixed state decoupling, spectral dimension(color imaging to hyperspectral imaging),high dynamic range,system extension,and typical applications. Among them,digital pathology is one of the earliest and the most successful applications of FPM. Distinguished from oth

关 键 词：数字病理学 全划片成像 计算成像 高通量 高内涵 傅里叶叠层显微成像术 

分 类 号：O438[机械工程—光学工程]