山羊CCNB2和ALDH1L2基因多态性与产羔性能的关联分析  

Association Analysis on Polymorphism of CCNB2 and ALDH1L2 Genes and Kidding Performance in Goats

作　　者：冯平捷 周李生 江炎庭 欧阳依娜 储明星[3] 洪琼花 FENG Pingjie;ZHOU Lisheng;JIANG Yanting;OUYANG Yina;CHU Mingxing;HONG Qionghua(Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]云南省畜牧兽医科学院,昆明650224 [2]青岛农业大学动物科技学院,青岛266109 [3]中国农业科学院北京畜牧兽医研究所,农业农村部动物遗传育种与繁殖重点实验室,北京100193

出　　处：《中国草食动物科学》2022年第5期16-21,共6页China Herbivore Science

基　　金：财政部和农业农村部-国家现代农业产业技术体系(CARS-38);云南省基础研究专项-重点项目(202001AS070002);云南省重大科技专项计划(202102AE090039);中国农业科学院科技创新工程(CAAS-ZDRW202106,ASTIP-IAS13)。

摘　　要：有报道显示,CCNB2与ALDH1L2基因与动物繁殖存在一定的联系,但关于他们在山羊繁殖中的作用还未清晰,为了改进山羊育种,提供有用的遗传标记,首先对3种山羊品种(济宁青山羊,辽宁绒山羊,云上黑山羊)768只母羊CCNB2基因和ALDH1L2基因的8个候选位点进行基因分型,之后再进行群体遗传和关联分析。结果表明,8个候选位点均存在多态性,CCNB2基因中除g.51957276C>T位点在济宁青山羊和辽宁绒山羊中为低度多态性(PIC<0.25)外,其余位点均处于中度多态性(0.25<PIC<0.5);ALDH1L2基因除g.67261430C>G位点在云上黑山羊和济宁青山羊群体中为中度多态性(0.25<PIC<0.5)外,其余位点均为低度多态性(PIC<0.25)。卡方检验结果表明,ALDH1L2基因大部分位点在3个群体中处于Hardy-Weinberg平衡状态(P>0.05),CCNB2基因g.51957276T>C位点在云上黑山羊和济宁青山羊群体中处于Hardy-Weinberg平衡状态(P>0.05),其余位点均处于Hardy-Weinberg不平衡状态(P<0.05)。在云上黑山羊中ALDH1L2基因g.67260707A>T位点TT型个体的产羔数显著高于AA型个体,在窝重上则没有显著性。综上所述,ALDH1L2基因的g.67260707A>T位点的突变可作为云上黑山羊产羔数选择的潜在分子标记。It has been reported that CCNB2 and ALDH1L2 genes are related to animal reproduction,but their role in goat reproduction is not clear.In order to improve goat breeding and provide useful genetic markers,this study first genotyped 8 candidate loci of CCNB2 and ALDH1L2 genes in 768 ewes of three goat breeds(Jining Grey goat,Liaoning Cashmere goat,Yunshang Black goat),and then carried out population genetic and association analysis.The results showed that there were polymorphisms in all 8 candidate loci.In CCNB2 gene,except for g.51957276C>T locus,which was low polymorphism(PIC<0.25)in Jining Grey goat and Liaoning Cashmere goat,the rest were moderate polymorphism(0.25<PIC<0.5);In ALDH1L2 gene,except for the g.67261430C>G locus was moderately polymorphic(0.25<PIC<0.5)in Yunshang Black goat and Jining Grey goat population,the rest were low polymorphism(PIC<0.25).Chi-square test showed that most loci of ALDH1L2 gene were in Hardy-Weinberg equilibrium(P>0.05)in the three populations;However,for the locus of CCNB2 gene,except g.51957276T>C was Hardy-Weinberg equilibrium(P>0.05)in the Yunshang Black goat and Jining Grey goat populations,the rest were in Hardy-Weinberg disequilibrium(P<0.05).In Yunshang Black goats,the litter size of individuals with TT type of ALDH1L2 gene g.67260707A>T was significantly higher than that of AA type,but there was no significant difference in litter weight.To sum up,the mutation of g.67260707A>T in ALDH1L2 gene can be used as a potential molecular marker for litter size selection in Yunshang Black goats.

关 键 词：山羊 产羔数 CCNB2基因 ALDH1L2基因 关联分析 

分 类 号：S827.2[农业科学—畜牧学]