鹿茸提取物对UVB诱导光老化小鼠皮肤的保护作用  

Protective effect of pilose antler extracts against UVB-induced skin photoaging in mice

作　　者：唐靖惠 王毅[2] 马淑骅[2] 孙娅楠[2] 贺改英 何忠梅[1] Jinghui Tang;Yi Wang;Shuhua Ma;Yanan Sun;Gaiying He;Zhongmei He(College of Chinese Medicine Materials,Jilin Agricultural University,Changchun,Jilin 130118,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国中医科学院医学实验中心,北京100700

出　　处：《日用化学工业》2022年第9期969-974,共6页China Surfactant Detergent & Cosmetics

基　　金：中央级公益性科研院所基本科研业务费(ZZ2018018,XTCX2021002);北京自然科学基金面上项目(7192141)。

摘　　要：研究鹿茸提取物(PAEs)对UVB诱导光老化小鼠皮肤的保护作用。通过HE染色和含水量测试仪检测光老化小鼠皮肤形态和含水量变化,采用双光子激发荧光(TPEF)和液晶偏光影像技术研究小鼠皮肤胶原纤维病理变化,RT-qPCR方法分析不同类型胶原蛋白、基质金属蛋白酶及基质金属蛋白酶抑制剂的表达。结果发现PAEs显著抑制UVB引起的小鼠皮肤厚度,增加光老化小鼠皮肤角质层和深层含水量。PAEs明显提高光老化小鼠真皮胶原纤维荧光信号强度,降低胶原纤维方位角的变异程度,促进光老化小鼠真皮Ⅰ型和Ⅲ型胶原蛋白mRNA表达,调控MMP-1和TIMP-2 mRNA表达。结果表明PAEs可以明显保护UVB引起皮肤的光老化,在抗衰老产品中具有重要的应用价值。Skin aging is an irreversible physiological process.Many factors,such as long-term UVB radiation,can accelerate skin aging and lead to a series of skin problems,whose symptoms include irregular pigmentation,wrinkles,dryness,loss of elasticity and capillary dilation.Herein,the protective effects of pilose antler extracts(PAEs)on skin photoaging induced by UVB in mice were investigated.The changes of epidermal thickness in photoaged skin were observed by H&E staining,while the changes of skin water content in photoaged mice were detected by a stratum corneum water content tester and a subcutaneous 0.05 mm water content tester.The fluorescence signal intensity of collagen fibers was detected in vivo by two-photon excitation fluorescence(TPEF)imaging technique,while the azimuth angle changes of collagen fibers were studied by liquid crystal polarized light imaging.RT-qPCR was used to analyze the expression of different types of collagen,matrix metalloproteinases and matrix metalloproteinase inhibitors.The results showed that,PAEs significantly inhibited UVB-induced hyperproliferation in mouse skin and increased the water content in the stratum corneum and deep layers of photoaged mouse skin.PAEs significantly increased the fluorescence signal intensity of dermal collagen fibers in photoaged mice,decreased the variability of collagen fiber azimuth,promoted the expression of dermal type I and III collagen mRNA in photoaged mice,and regulated MMP-1 and TIMP-2 mRNA expression.The experimental results showed that PAEs could significantly improve UVB-induced skin photoaging and have important applications in antiaging products.

关 键 词：鹿茸提取物 光老化 胶原 基质金属蛋白酶 

分 类 号：TQ658[化学工程—精细化工]