硅烷化玻璃酸酯对皮肤屏障相关基因和蛋白的作用研究  

Effects of silanol hyaluronic acid compound (SHAC) on skin barrier genes and proteins

作　　者：王晓娜 韩婷婷 郭芳钰 王兴凯 郭海姣 陈建英[2] 李燕 陈玉荣 杨素珍 WANG Xiao-na;HAN Ting-ting;GUO Fang-yu;WANG Xing-kai;GUO Hai-jiao;CHEN Jian-ying;LI Yan;CHEN Yu-rong;YANG Su-zhen(Shandong Freda Biotech Co.,Ltd.,Jinan,Shandong 250101,China;Provincial Key Laboratory of Biology and Pharmacology,Biopharmaceutical Institute of Shandong Province,Jinan,Shandong 250101,China)

机构地区：[1]山东福瑞达生物股份有限公司,山东济南250101 [2]山东省生物药物研究院山东生物药物重点实验室,山东济南250101

出　　处：《日用化学品科学》2022年第9期20-24,33,共6页Detergent & Cosmetics

摘　　要：主要通过qRT-PCR及免疫荧光(IF)法,研究了硅烷化玻璃酸酯(SHAC)对于皮肤屏障相关的8种基因表达和2种屏障相关蛋白合成的影响。选取合适样品浓度检测培养24 h后角质形成细胞中的FLG、LOR、IVL、KRT10、KRT16、TGM1、CLDN1、OCLN 8种基因表达量,同时检测角质形成细胞中ZO-1、CLDN1 2种蛋白的表达量。结果表明,SHAC在浓度0.625%时能显著促进IVL、TGM1、OCLN、CLDN1基因的表达,在浓度为2.5%时能显著促进OCLN、TGM1基因的表达;当SHAC浓度在0.625%时,能更好促进CLDN1基因的表达并能显著促进CLDN1蛋白含量上升,对ZO-1蛋白作用不明显,SHAC具有潜在的屏障修护作用。The effects of silanol hyaluronic acid compound (SHAC) on the expression of 8 skin barrier related genes and the synthesis of 2 skin barrier related proteins were studied by qRT-PCR and immunofluorescence methods.The expression levels of FLG,LOR,IVL,KRT10,KRT16,TGM1,CLDN1 and OCLN genes in keratinocytes after 24 h culture were detected at appropriate sample concentration,and the expression levels of ZO-1 and CLDN1 proteins in keratinocytes were also detected.The results showed that the expression of IVL,TGM1,OCLN and CLDN1 could be significantly promoted at an SHAC concentration of 0.625%,while the expression of OCLN and TGM1 could be significantly promoted at an SHAC concentration of 2.5%.Especially when SHAC concentration was 0.625%,CLDN1 gene expression could be significantly promoted.When SHAC concentration was 0.625%,CLDN1 protein content was significantly increased,but no obvious effect was observed for ZO-1 protein.The results show that SHAC has potential barrier repair function.

关 键 词：硅烷化玻璃酸酯 透明质酸钠 紧密连接蛋白 CLDN1 OCLN 屏障修护 

分 类 号：TQ658[化学工程—精细化工]