葡萄根癌病研究进展及防治措施  

Advances and Prevention Measures in Grape Crown Gall

作　　者：袁丽芳 尹向田 王鹏飞 李廷刚 蒋锡龙 汤小宁 韩金涛 魏彦锋 YUAN Lifang;YIN Xiangtian;WANG Pengfei;LI Tinggang;JIANG Xilong;TANG Xiaoning;HAN Jintao;WEI Yanfeng(Shandong Academy of Grape,Jinan 250100,China;Shandong Academy of Pesticide Sciences,Jinan 250100,China)

机构地区：[1]山东省葡萄研究院,山东济南250100 [2]山东省农药科学研究院,山东济南250100

出　　处：《中外葡萄与葡萄酒》2022年第5期93-99,共7页Sino-Overseas Grapevine & Wine

基　　金：山东省农业科学院农业科技创新工程(CXGC2022E15,CXGC2022A13,CXGC2021A25);山东省自然科学基金青年项目(ZR2021QC131);山东省葡萄研究院引导基金(SDAG2021B06,SDAG2021B02,SDAG2021B10)。

摘　　要：葡萄根癌病由葡萄土壤杆菌(Allorhizobium vitis)引起,主要通过伤口侵染,促使植物细胞产生过量的生长激素,从而形成瘤状物,影响植株的营养吸收,进而降低葡萄产量和品质。本文综述了葡萄根癌病的发病症状、病原菌种类、病原菌的基因组信息及分类地位,比较分析了基于特异性引物建立的葡萄土壤杆菌的PCR、荧光定量PCR、数字微滴PCR、环介导等温扩增技术等分子生物学检测技术的优缺点,阐述了病原菌的发病机制及对该病的综合防治方法,为葡萄根癌病的后期研究及生产防治提供参考。Grape crown gall is caused by Allorhizobium vitis,mainly survived in soil and infected plants by wound,prompting plant cells to produce excessive growth hormone,thus forming tumors,affecting the nutrient absorption of plants,and then reducing the yield and quality of grapes.The symptoms,pathogenic bacteria and their classification status were reviewed in this paper,the advantages and disadvantages of detection technologies,including PCR,real-time PCR,droplet digital PCR and loop-mediated isothermal amplification were compared,and pathogenesis and comprehensive control methods of grape crown gall were elaborated,which could provide a foundation for later research and control of grape crown gall.

关 键 词：葡萄根癌病 鉴定 检测 发病机制 综合防治 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]