耐铅链霉菌诱导碳酸钙沉淀对水中Pb^(2+)的去除  

Removal of Pb^(2+)from Water by Pb-tolerant Streptomyces-induced Precipitation of Calcium Carbonate

作　　者：苟渔 牛丽娟 赵梦琦 苏欢 郭飞 李丽[2] GOU Yu;NIU Lijuan;ZHAO Mengqi;SU Huan;GUO Fei;LI Li(College of Life Sciences and Technology,Xinjiang University,Urumqi 830017,China;Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830017 [2]中国科学院新疆生态与地理研究所,乌鲁木齐830011

出　　处：《有色金属工程》2022年第9期165-172,共8页Nonferrous Metals Engineering

基　　金：新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划及国际科技合作计划)(2020E01045);自治区高校科研计划自然科学项目(XJEDU2019Y004)。

摘　　要：微生物诱导碳酸钙沉淀(MICP)可将水中游离的Pb^(2+)转化为稳定的沉淀物。筛选出一株耐Pb^(2+)的脲酶阳性SHT17菌株,16S rRNA鉴定为Streptomyces mutabilis(登录号为OL677072)。探究该链霉菌诱导碳酸钙沉淀对水中Pb^(2+)的去除能力。通过电感耦合等离子体发射光谱仪(ICP-OES)对水中Pb^(2+)浓度进行检测,利用扫描电子显微镜(SEM)、X-射线能谱(EDS)、X-射线衍射(XRD)和傅里叶红外光谱(FTIR)对菌株诱导的沉淀物进行分析。结果表明,链霉菌能有效地去除水中Pb^(2+)。当Pb^(2+)浓度为200 mg/L时,水中Pb^(2+)去除率达到99.66%,并且去除的Pb^(2+)以碳酸盐的形式被固定在链霉菌诱导生成的沉淀物中。此外,随着初始Pb^(2+)浓度升高,该菌对Pb^(2+)去除率均在65%以上,且大致呈下降趋势。不同钙源影响着链霉菌诱导形成的沉淀物的形貌。Microbially induced calcium carbonate precipitation(MICP)can transform free lead ions into stable precipitation.In this study,a lead-tolerant and urease-positive SHT17 strain was screened,which was identified as Streptomyces mutabilis(Accession No.OL677072)by 16S rRNA sequences.The removal ability of Pb^(2+) from water was researched by Streptomyces-induced precipitation of calcium carbonate.The Pb^(2+) concentration was measured by inductively coupled plasma optical emission spectrometry(ICP-OES).The microbial-induced precipitation was analyzed by scanning electron microscopy(SEM),X-ray energy distribution(EDS),X-ray diffraction(XRD)and fourier transform infrared spectroscopy(FTIR).The results showed that Streptomyces mutabilis possessed the highest Pb^(2+) removal ability.The Pb^(2+) removal rate reached 99.66%(with initial Pb^(2+) concentration 200 mg/L),and removed Pb^(2+) was fixed in the Streptomyces-induced carbonate precipitation.Furthermore,with the increase of the initial Pb^(2+) concentration,the removal rate of Pb^(2+) was above 65%,and showed a general downward trend.Different calcium sources affect the morphology of the Streptomyces-induced precipitations.

关 键 词：MICP 铅污染 碳酸钙 链霉菌属 

分 类 号：Q939.99[生物学—微生物学]