五味子煎剂对貉源大肠杆菌体外增殖和耐药性的影响  

Effect of Schisandra Decoction on in vitro Proliferation and Drug Resistance of Escherichia coli from Raccoon Dog

作　　者：王婧文 谭贻鸿 高昊天 张东林[1] 史秋梅[1] 高桂生[1] 高光平[1] WANG Jing-wen;TAN Yi-hong;GAO Hao-tian;ZHANG Dong-lin;SHI Qiu-mei;GAO Gui-sheng;GAO Guang-ping(Hebei Normal University of Science&Technology,Key Laboratory of Preventive Veterinary Medicine of Hebei Province,Qinhuangdao 066004,China;Beijing University of Chinese Medicine DongFang College,Langfang 065001,China)

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北秦皇岛066004 [2]北京中医药大学东方学院,河北廊坊065001

出　　处：《中国兽医杂志》2022年第7期105-110,共6页Chinese Journal of Veterinary Medicine

基　　金：秦皇岛市科技局项目(201903B007);河北省第三批巨人计划—中草药防治畜禽水产养殖动物传染病科研创新团队;河北科技师范学院科学研究基金。

摘　　要：为了研究不同浓度的五味子煎剂对貉源大肠杆菌体外增殖和耐药性的影响,本试验选用500.00、250.00、125.00、62.50、31.25、15.63、7.81、3.91、1.95 mg/mL和0.98 mg/mL共10个浓度梯度的五味子煎剂分别作用于貉源大肠杆菌4、8、12、16、20 h和24 h,然后用琼脂平板培养计数法检测貉源大肠杆菌体外增殖情况,用PCR法检测貉源大肠杆菌耐药基因的变化,并用K-B法检测五味子煎剂作用后貉源大肠杆菌对抗菌药的敏感性。结果显示,15.63 mg/mL及以上浓度的五味子煎剂对貉源大肠杆菌有明显的抑制作用,并且31.25 mg/mL的五味子煎剂对貉源大肠杆菌耐药基因消除效果较强。结果表明,15.63 mg/mL及以上浓度的五味子煎剂可抑制貉源大肠杆菌体外增殖,并且可消除菌株的耐药性,在养殖过程中可用于预防和治疗貉大肠杆菌病。This study investigated the effect of different concentrations of Schisandra decoction on the in vitro proliferation and drug resistance of Escherichia coli from raccoon dog.Schisandra decoction at concentrations of 500.00,250.00,125.00,62.50,31.25,15.63,7.81,3.91,1.95 mg/mL and 0.98 mg/mL was applied to E.coli from raccoon dog for 4,8,12,16,20 h and 24 h.The in vitro proliferation of E.coli from raccoon dog was then detected by agar plate culture counting,the change of E.coli resistance genes was determined by PCR,and the susceptibility of E.coli drug resistance was measured by K-B method.The results showed that Schisandra decoction at concentrations of 15.63 mg/mL and above had strong inhibitory effect on E.coli from raccoon dog,and Schisandra decoction at concentration of 31.25 mg/mL had good effect on E.coli drug resistance gene elimination.Thus,Schisandra decoction at concentrations of 15.63 mg/mL and above could inhibit the proliferation of and eliminate drug resistance in E.coli from raccoon dog in vitro,and Schisandra decoction should be considered for treatment and prevention colibacillosis in raccoon dog during breeding.

关 键 词：五味子 貉 大肠杆菌 增殖 耐药性 

分 类 号：R378.21[医药卫生—病原生物学]