Rapid and sensitive detection of Oncorhynchus mykiss adulteration in Salmo salar by recombinase polymerase amplification combined with lateral flow strip  

作　　者：Qiankun Yang Xia Liu Lei Wang Guili Yu Haitao Yang Hang Fu Xueqing Li Nana He Hong Yu Jingquan Dong 

机构地区：[1]Jiangsu Key Laboratory of Marine Bioresources and Environment,Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,Jiangsu Ocean University,Lianyungang,222005,China [2]Department of Laboratory Medicine,the Second People’s Hospital of Lianyungang City,Lianyungang 222000,China [3]School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212018,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第7期1039-1042,共4页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the Basic Science(Natural Science)Research Project of Higher Education of Jiangsu Province(No.21KJB230001);the Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening(No.HY202101);the Priority Academic Program Development of Jiangsu Higher Education Institutions of China;the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.SY202112X).

摘　　要：Oncorhynchus mykiss,a kind of freshwater fish,belongs to the Salmonidae family and Oncorhynchus genus[1].O.mykiss is farmed in more than 20 cities in China,being one of the most common cold-water farming fish.The wide range of O.mykiss may attribute to its rapid growth and tolerance of different environmental conditions.Salmo salar attracts customers’interest for its delicious taste and high nutrient contents.However,wild S.salar supply is far less than high demands,and artificial breeding faces difficulties in yield and cost.External morphological characteristics can distinguish full-fledged fish,but it is difficult to distinguish after processing with pigments for the white and blue O.mykiss meat displaying orange color and texture[2].The food industry provides cheaper O.mykiss adulteration in S.salar,infringing consumers’rights and interests.Moreover,farmed O.mykiss in fresh water may risk parasite infection,and customers who eat raw food face food safety concerns[3].

关 键 词：SALMO MYKISS breeding 

分 类 号：S91[农业科学—水产科学]