花生种子大小相关性状QTL定位及与出仁率的关系  

QTL Mapping for Seed Size Related Traits and Its Relationship with Shelling Percentage in Peanut

作　　者：郭建斌[1] 李威涛 罗怀勇[1] 陈伟刚[1] 喻博伦 黄莉[1] 刘念[1] 周小静[1] 姜慧芳[1] GUO Jian-Bin;LI Wei-Tao;LUO Huai-Yong;CHEN Wei-Gang;YU Bo-Lun;HUANG Li;LIU Nian;ZHOU Xiao-Jing;JIANG Hui-Fang(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs,Wuhan 430062)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,武汉430062

出　　处：《植物遗传资源学报》2022年第5期1465-1473,共9页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31971903);农作物种质资源保护项目(2019NWB033);国家农作物种质资源共享服务平台(NICGR2021-016);国家花生产业技术体系建设(CARS-13-种质资源评价)。

摘　　要：花生是我国重要的油料作物之一,高产是花生育种的重要目标性状,种子大小和出仁率是花生产量构成的因素。定位种子大小性状相关QTL,并分析其与出仁率的关系,将为花生高产分子育种奠定基础。本研究以种子大小差异显著的徐花13和中花6号为亲本构建的重组自交系群体(RIL,recombinant inbred line)为材料,连续3年对种子大小性状进行考察,种子长、种子宽和百仁重在RIL群体中变异广泛,并且性状间均极显著正相关。通过前期构建的遗传图谱,共检测到52个QTL位点,贡献率为3.09%~17.34%,其中qSLA05.2和qHSWA05.2,qSWA07.1和qHSWA07为两个性状共定位的主效QTL并且能在多个环境下被重复检测到。qHSWA05.2和qHSWA07的有利等位基因分别来自于母本和父本,利用连锁标记发现这两个有利等位基因组合在RIL群体中能使百仁重平均增加33.80 g。结合前期利用该群体出仁率QTL定位结果,发现出仁率与种子大小主效QTL除存在共定位外,也存在特有QTL。上述结果为精细定位和分子标记辅助育种以及多性状协同改良花生产量提供了重要信息。Peanut is one of the important oilseed crops in China.Breeding for high yield is an important target.Seed size and shelling percentage are the factors effecting of peanut yield.QTL mapping for seed size and analyzing its relationship with shelling percentage will lay a foundation for high yield molecular breeding.In this study,the seed size of a recombinant inbred line(RIL)population(Xuhua 13×Zhonghua 6)were investigated at three consecutive years.The seed length(SL),seed width(SW),and hundred seed weight(HSW)were variable in RIL population,and a significant positive correlation was detected among them.52 QTL were detected with 3.09%-17.34%phenotypic variations explained(PVE),of which qSLA05.2 and qHSWA05.2,qSWA07.1 and qHSWA07 are co-localized and they can be repeatedly detected in multiple environments.Favorable alleles of qHSWA05.2 and qHSWA07 were derived from the female and male parents,respectively.A combination of the two favorable alleles using the linked markers was verified to average increase hundred seed weight by 33.80 g in the RIL population.In conjugation with the QTL mapping result of shelling percentage,co-localized QTL and specific QTL for either trait were found.Collectively,this study laid a foundation for future fine mapping,molecular marker-assisted breeding and synergistic improvement of high yield in peanut.

关 键 词：花生 种子大小 出仁率 QTL 

分 类 号：S565.2[农业科学—作物学]