广东2020-2021年乙型流感病毒分子变异特征  

Molecular mutation features of influenza B viruses in Guangdong,2020-2021

作　　者：谭静 梁丽君[2] 黄平 黄晓玲[2] 李柏生 曹虹[1] 邹丽容[2] TAN Jing;LIANG Lijun;HUANG Ping;HUANG Xiaoling;LI Baisheng;CAO Hong;ZOU Lirong(School of Public Health,Southern Medical University,Guangzhou,Guangdong Province,510515;Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,Guangdong Province,511430,China)

机构地区：[1]南方医科大学公共卫生学院,广州510515 [2]广东省疾病预防控制中心广东新发突发传染病防治工作站,广州511430

出　　处：《陆军军医大学学报》2022年第17期1791-1796,F0003,共7页Journal of Army Medical University

基　　金：广东省自然科学基金项目(2016A030313775);广州市科技计划项目(201904010286);广东省中医药局科研项目(20202024)。

摘　　要：目的了解乙型流感Victoria株系(influenza B victoria lineage,Bv)疫情的分子基础,分析该株系流感的流行趋势。方法基于广东流感监测网络(GDISN)采用时空分布方法筛选流感Bv毒株,检测HA/NA基因核苷酸序列;采用MEGA 7.026、Datamonkey 2.0、NetNGlyc 1.0 Server、SPSS 23.0软件等,分析其核苷酸/氨基酸序列的同源性、进化树、氨基酸与表位变异、糖基化位点和进化选择性等。结果广东2021年毒株较2020年毒株同源性呈现明显差异(P<0.01),2021年下半年毒株(17株)HA基因与疫苗株B/Austria/1359417/2021同源性高达(99.28±0.25)%。HA基因A表位(HN/Q、AT和RG)、B表位(PL和NK)和D表位(SN/D和TA)以及RBS位点(PQ)发生变异;2020年毒株呈Bv.1a.3株系特征,2021年毒株主要呈Bv.1a.3a.2株系特征。HA与NA基因潜在糖基化位点均无有意义改变;NA基因没有出现药敏位点变异;HA和NA基因氨基酸负向选择位点分别是73、138、437和497位点和179、321和335位点。结论广东2021年毒株HA和NA基因较2020年毒株均发生较明显变异,尤以2021年下半年毒株的HA基因变异明显;Bv毒株HA基因表位出现新变异,提示引发下一流感季节广泛局部流行的预警。Objective To learn the molecular characteristics of influenza B Victoria lineage(Bv)and evaluate the influenza B spread trend about the epidemic.Methods Based on the Guangdong Influenza Surveillance Networks(GDISN)to selected the Bv with space-time sampling,the nucleotide sequences of HA/NA genes were detected.The evolution and mutations of both genes were analyzed in homology with MEGA 7.026和SPSS 23.0,in evolutionary tree with neighbor-joining method,in selective evolution with Datamonkey 2.0.Results The nucleotide homologies of HA/NA genes in the 2021 strains were statistically different from these of the 2020's(P<0.01),in which the those of HA genes in 17 strains isolated in the second half of 2021 were up to(99.28±0.25)%compared with these in the vaccine strain B/Austria/1359417/2021.As to HA genes,the epitope A(H137N/Q,A142T and R148G),B(P159L and N162K)and D(S209N/D and T211A)were mutated,therefore the 2020 and 2021 strains in Guangdong were respectively classified into Clade Bv.1a.3 and Bv.1a.3a.2.There were significantly no mutations in the potential glycosylation sites in HA/NA genes,as well in the NA known drug sensitive sites.Evolutionary selection conducted the negative selection sites in HA/NA genes,including amino acid sites 73,138,437 and 497 in HA and sites 179,321 and 335 in NA.Conclusion Influenza Bv strains including HA and NA genes in Guangdong 2021 strain are significantly different from these of the 2020 strains,especially on HA genes in the second half of 2021.

关 键 词：乙型流感 乙型Victoria株系(Bv) 进化 表位 负向选择 

分 类 号：R195.4[医药卫生—卫生统计学] R394.3[医药卫生—卫生事业管理] R511.7[医药卫生—公共卫生与预防医学]